Differential effects of plant growth regulators on physiology, steviol glycosides content, and antioxidant capacity in micropropagated tissues of Stevia rebaudiana

Basit Öğe Kaydı
dc.authorid0000-0002-6786-3973
dc.authorid0000-0001-6262-2866
dc.contributor.authorRabia Javed
dc.contributor.authorYücesan, Bahtiyar Buhara
dc.contributor.authorZia M.
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T18:51:36Z
dc.date.available2021-06-23T18:51:36Z
dc.date.issued2017
dc.departmentBAİBÜ, Ziraat Fakültesi, Tohum Bilimi ve Teknolojisi Bölümüen_US
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractPlants naturally produce secondary metabolites on exposure to abiotic/biotic stress. Production of secondary metabolites, like phenols or flavonoids, is a defence mechanism against different stresses. This study explores the influence of plant growth regulators (PGRs) on the growth and secondary metabolites of in vitro grown tissues of zero calorie sweetener, Stevia rebaudiana. An efficient regeneration of this valuable plant under the influence of PGRs was conducted, and quantification of physiology parameters, steviol glycosides (SGs) and various antioxidant activities in callus, shoots and regenerated plants was performed. Significantly highest explant response was achieved by 2 mg/L of 6-benzyl aminopurine (BAP) and 0.5 mg/L of 2,4-dichlorophenoxy acetic acid (2,4-D) combination in case of callogenesis, while shoot and root organogenesis was best illustrated by control and 0.5 mg/L of indole acetic acid (IAA) treatment, respectively. Regarding SGs formation in leaves, significantly greater content (2.07% and 0.93%) was obtained by control in case of rebaudioside A (Reb A) and 2 mg/L of BAP in case of stevioside (ST). Leaves of rooted-shoots produced significantly highest Reb A (4.21%) and ST (2.22%) content in case of 1 mg/L of IAA and 0.25 mg/L of IAA inoculation in Murashige and Skoog growth medium, respectively. Similarly, differential effects of PGRs were elucidated in case of all in vitro grown tissues on antioxidant properties. The study concludes that secondary metabolites can be efficiently produced in in vitro culture so can be employed in bioreactors. © 2017 Institute of Molecular Biology, Slovak Academy of Sciences 2017.en_US
dc.description.sponsorship2216 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAKen_US
dc.description.sponsorshipRabia Javed is grateful to The Scientific and Technological Research Council of Turkey, TUBİTAK (Programme No: 2216) for providing financial support to conduct this research. Authors are also thankful to the Department of Biotechnology, Quaid-i-Azam University, Pakistan, and Department of Biology, Abant Izzet Baysel University, Turkey, for providing all research facilities.en_US
dc.identifier.doi10.1515/biolog-2017-0133
dc.identifier.endpage1165en_US
dc.identifier.issn0006-3088
dc.identifier.issue10en_US
dc.identifier.scopus2-s2.0-85035815982en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1156en_US
dc.identifier.urihttps://doi.org/10.1515/biolog-2017-0133
dc.identifier.urihttps://hdl.handle.net/20.500.12491/3893
dc.identifier.volume72en_US
dc.identifier.wosWOS:000416046200007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorYücesan, Bahtiyar Buhara
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherDe Gruyter Open Ltden_US
dc.relation.ispartofBiologia (Poland)en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAsteraceaeen_US
dc.subjectcallusen_US
dc.subjectorganogenesisen_US
dc.subjectphysiologyen_US
dc.subjectStevia rebaudianaen_US
dc.subjectsteviol glycosidesen_US
dc.titleDifferential effects of plant growth regulators on physiology, steviol glycosides content, and antioxidant capacity in micropropagated tissues of Stevia rebaudianaen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
rabia-javed2.pdf
Boyut:
902.51 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam metin/ Full text
İndir

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
Biyoloji Bölümü Koleksiyonu
Tohum Bilimi ve Teknolojisi Bölümü Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu