Practical identification of human originated Lactobacillus species by amplified ribosomal DNA restriction analysis (ARDRA) for probiotic use

dc.authorid0000-0001-6906-5257
dc.contributor.authorÖztürk, Mehmet
dc.contributor.authorMeterelliyöz, Merve
dc.date.accessioned2021-06-23T19:41:59Z
dc.date.available2021-06-23T19:41:59Z
dc.date.issued2015
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractProbiotics are gaining popularity and increasing the importance of their accurate speciation. Lactobacillus species are commonly used as probiotic strains mostly of clinical importance. Present knowledge indicates that at least 14 Lactobacillus species are associated with the human intestinal tract. Currently, researchers are interested in developing efficient techniques for screening and selecting probiotics bacteria, but unfortunately most of these methods are time-consuming, labor-intensive and costly. The aim of this study is to develop reliable, rapid and accurate method to identify 14 references Lactobacillus species that could have been found in the human alimentary tract by 16S ribosomal DNA restriction analysis. In this study, to develop an effective method for the genotype-based identification of the reference Lactobacillus species, 1.5 kb of 16S rRNA nucleotide sequences of 14 Lactobacillus were collected from the Gene Bank aligned, in silico restricted and analyzed in respect to their 16S-rRNA restriction fragment polymorphism. In silico restriction profiles of 16S-rRNA indicated that FspBI, HinfI and DraI restriction enzymes (RE) are convenient for differentiation of 14 Lactobacillus species in human intestinal tract except Lb. casei and Lb. paracasei. The patterns of our experimental findings obtained from 16S PCR-ARDRA completely confirmed our in silico patterns. The present work demonstrated that 16S PCR-ARDRA method with FspBI, HinfI and DraI RE is a rapid, accurate and reliable method for the identification of Lactobacillus species from human alimentary tract, especially during the identification of large numbers of isolates and any laboratory equipped with a thermo cycler for probiotic use.en_US
dc.identifier.doi10.1007/s11033-015-3877-7
dc.identifier.endpage1332en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue8en_US
dc.identifier.pmid25860079en_US
dc.identifier.scopus2-s2.0-84937825957en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1323en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-015-3877-7
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8271
dc.identifier.volume42en_US
dc.identifier.wosWOS:000358165300010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÖztürk, Mehmet
dc.institutionauthorMeterelliyöz, Merve
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reportsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectProbiotic Strainsen_US
dc.subjectLactobacillus Speciesen_US
dc.subjectAmplified Ribosomal DNA Restriction Analysis (ARDRA)en_US
dc.subject16S-rRNA Geneen_US
dc.titlePractical identification of human originated Lactobacillus species by amplified ribosomal DNA restriction analysis (ARDRA) for probiotic useen_US
dc.typeArticleen_US

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