The effects of hypericin on ADAMTS and p53 gene expression in MCF-7 breast cancer cells

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dc.authorid0000-0002-1012-001Xen_US
dc.authorid0000-0003-4357-5229en_US
dc.authorid0000-0002-6839-2632
dc.contributor.authorAcar, Muradiye
dc.contributor.authorOcak, Zeynep
dc.contributor.authorErdoğan, Kübra
dc.contributor.authorÇetin, Elif Nihan
dc.contributor.authorHatipoğlu, Ömer Faruk
dc.contributor.authorÜyetürk, Ümmügül
dc.date.accessioned2021-06-23T19:35:44Z
dc.date.available2021-06-23T19:35:44Z
dc.date.issued2014
dc.departmentBAİBÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractPurpose: The purpose of this study was to determine the effects of hypericin on MCF-7 (Michigan Cancer Foundation-7) breast cancer cells, as it is known to exert an antitumor effect on the expression and regulation of ADAMTS1, 3, 10 and the p53 gene in breast cancer cells. Methods: MFC-7 cells were cultured and subjected separately to various doses (1, 5 and 7.5 mu g/mL) hypericin. After 24 hrs, RNA was isolated and transcribed into cDNA. Expression analysis was performed by real time (RT)-PCR and cell survival was determined by the XTT assay. Results: While the expression of ADAMTS1 in MFC-7 cells decreased to 0.04-fold after exposure to 1 mu g/mL hypericin, the expression increased by 5.6- and 36-fold with 5 and 7.5 mu g/mL, respectively. Furthermore, ADAMTS3 expression in MCF7 cells increased 3.9-fold with the use of 5 mu g/mL of hypericin. These concentrations of hypericin did not lead to significant changes in the expression of ADAMTS10 and the p53 gene. Viability of cancer cells as evaluated by the XTT assay showed that hypericin concentration of 7.5 mu g/mL led to increased apoptosis of cancer cells. Conclusion: The increase in ADAMTS1 expression may prevent metastasis or facilitate the development of an adjuvant factor with tumor-suppressive effects. Hypericin may therefore exert its antitumor and apoptotic effects in MFC-7 cells via ADAMTS1 and ADAMTS3.en_US
dc.identifier.endpage632en_US
dc.identifier.issn1107-0625
dc.identifier.issn2241-6293
dc.identifier.issue3en_US
dc.identifier.pmid25261644en_US
dc.identifier.scopus2-s2.0-84908458556en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage627en_US
dc.identifier.urihttps://www.jbuon.com/archive/19-3-627.pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7857
dc.identifier.volume19en_US
dc.identifier.wosWOS:000342963300005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorOcak, Zeynep
dc.institutionauthorÜyetürk, Ümmügül
dc.language.isoenen_US
dc.publisherImprimatur Publicationsen_US
dc.relation.ispartofJournal Of Buonen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectADAMTSen_US
dc.subjectBreast Canceren_US
dc.subjectHypericinen_US
dc.subjectp53 Geneen_US
dc.titleThe effects of hypericin on ADAMTS and p53 gene expression in MCF-7 breast cancer cellsen_US
dc.typeArticleen_US

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