In vitro and ex vitro propagation of Stevia rebaudiana Bertoni with high Rebaudioside-A content-A commercial scale application

Basit Öğe Kaydı
dc.authorid0000-0001-6262-2866en_US
dc.authorid0000-0002-6786-3973
dc.authorid0000-0003-4740-1138
dc.contributor.authorYücesan, Buhara
dc.contributor.authorMohammed, Aliyu
dc.contributor.authorBüyükgöçmen, Refik
dc.contributor.authorAltuğ, Cevher
dc.contributor.authorKavas, Özge
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:43:34Z
dc.date.available2021-06-23T19:43:34Z
dc.date.issued2016
dc.departmentBAİBÜ, Ziraat Fakültesi, Tohum Bilimi ve Teknolojisi Bölümüen_US
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractStevia rebaudiana Bertoni is a self-incompatible and the pollination is an entomophilous short-day plant. Rebaudioside A, as an individual steviol glycoside, is of particular interest in global sweetener market due to its most desirable flavour profile as compared to stevioside having aftertaste bitterness. In the present investigation, regenerant formation was performed for 6 weeks, in which randomly selected nodes excised from in vitro germinated seedlings were cultured on solid Murashige and Skoog medium (MS) with or without growth regulators (6-benzylaminopurine- BAP or kinetin- KIN) at various concentrations (ranging from 0.1 to 2.0 mg/L). Irrespective of the medium composition, the results showed that all treatments were effective for shoot induction, producing an average of 2 shoots per explant after three weeks of culture. Following subsequent sub-culturing on MS medium at 3-week intervals, all the shoots regenerated from the nodes were transferred to MS medium with or without indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or naphthalene acetic acid (NAA) at two different concentrations (0.25 or 0.50 mg/L) for root formation for 3 weeks. IAA was more effective for root formation, producing 7.6 roots per shoot with 100% rooting frequency. All the regenerants (similar to 13.5 cm in length) were potted and successfully acclimatized in a greenhouse for 2 weeks, and then transferred to the field for 14 weeks with a high survival rate (>99%). Similarly, seedlings (8 weeks-old) derived from seed germination in pots were also transferred to the same field in a different plot. There was no significant difference in terms of morphological, yield performance and steviol glycoside compositions between regenerants and seedlings sampled from two developmental periods (late vegetative and flowering period). It was clearly shown that clonal propagation using nodal explants was effective for superior stock plant production with high Reb-A content (11.7% w/w). (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.scienta.2016.03.008
dc.identifier.endpage28en_US
dc.identifier.issn0304-4238
dc.identifier.issn1879-1018
dc.identifier.scopus2-s2.0-84960399208en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage20en_US
dc.identifier.urihttps://doi.org/10.1016/j.scienta.2016.03.008
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8807
dc.identifier.volume203en_US
dc.identifier.wosWOS:000375337200003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorYücesan, Buhara
dc.institutionauthorMohammed, Aliyu
dc.institutionauthorBüyükgöçmen, Refik
dc.institutionauthorAltuğ, Cevher
dc.institutionauthorKavas, Özge
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofScientia Horticulturaeen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectStevia Rebaudianaen_US
dc.subjectIn Vitro Regenerationen_US
dc.subjectRebaudioside Aen_US
dc.subjectSteviol Glycosidesen_US
dc.subjectSynseeden_US
dc.subjectHigh Intensive Sweetenersen_US
dc.titleIn vitro and ex vitro propagation of Stevia rebaudiana Bertoni with high Rebaudioside-A content-A commercial scale applicationen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
1-s2.0-S0304423816301145-main.pdf
Boyut:
2.94 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Biyoloji Bölümü Koleksiyonu
Kimya Bölümü Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Tohum Bilimi ve Teknolojisi Bölümü Koleksiyonu