Investigation of the effects of methylphenidate, an amphetamine derivative, on ıntervertebral disc tissue cell cultures and matrix structures

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dc.authorid0000-0002-5412-8355
dc.authorid0000-0001-5590-0637
dc.authorid0000-0002-1224-442X
dc.authorid0000-0002-8084-7855
dc.authorid0000-0001-5672-0566
dc.contributor.authorKaya, Yasin Emre
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorSirin, Duygu Yaşar
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorKaplan, Necati
dc.date.accessioned2021-06-23T18:40:36Z
dc.date.available2021-06-23T18:40:36Z
dc.date.issued2019
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractAIM: To investigate the effects of methylphenidate (MPH), on intervertebral disc tissue (IVD) cell cultures and extracellular matrix structures. Changes in the expression of some important marker genes involved in anabolic and catabolic mechanisms of IVD extracellular matrix formation were also evaluated. MATERIAL and METHODS: Primary cultures of nucleus pulposus cells (NPCs) and annulus fibrosus cells (AFCs) were isolated from tissues obtained from the operated patients. Cell viability and proliferation were tested, and the cell surface morphologies were evaluated by microscopy. The expressions of the chondroadherin (CHAD), cartilage oligomeric matrix protein (COMP), interleukin-1 beta (IL-1β) and matrix metalloproteinase (MMP) -7 and MMP-19 genes were evaluated using the quantitative real-time polymerase chain reaction (qRT-PCR). A value of p<0.05 was considered statistically significant. RESULTS: The viability and proliferation of intervertebral disc tissue cells decreased in response to MPH treatment and the expression of the investigated genes also changed. CONCLUSION: The data obtained from in-vitro studies may not directly adaptable to clinical applications. However, the fact that the central nervous system stimulant MPH can suppress proliferation of cells derived from IVD tissue should be considered carefully by clinicians.en_US
dc.identifier.doi10.5137/1019-5149.JTN.26339-19.2
dc.identifier.endpage742en_US
dc.identifier.issn1019-5149
dc.identifier.issn2651-5032
dc.identifier.issue5en_US
dc.identifier.pmid31353439en_US
dc.identifier.scopus2-s2.0-85072350718en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage734en_US
dc.identifier.trdizinid356361en_US
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.26339-19.2
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TXpVMk16WXhNUT09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/3163
dc.identifier.volume29en_US
dc.identifier.wosWOS:000486326100017en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaya, Yasin Emre
dc.language.isoenen_US
dc.relation.ispartofTurkish Neurosurgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCartilage Oligomeric Matrix Proteinen_US
dc.subjectChondroadherin
dc.subjectInterleukin-1 Beta
dc.subjectIntervertebral Disc
dc.subjectMatrix Metalloproteinase
dc.subjectMethylphenidate
dc.titleInvestigation of the effects of methylphenidate, an amphetamine derivative, on ıntervertebral disc tissue cell cultures and matrix structuresen_US
dc.typeArticleen_US

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