The effect of sub-chronic systemic ethanol treatment on corpus cavernosal smooth muscle contraction: the contribution of RhoA/Rho-kinase

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dc.authorid0000-0003-3691-208Xen_US
dc.authorid0000-0003-3316-7002en_US
dc.authorid0000-0003-0224-0158
dc.contributor.authorKumcu, Eda Karabal
dc.contributor.authorAydınoğlu, Fatma
dc.contributor.authorAstarcı, Erhan
dc.contributor.authorÖğülener, Nuran
dc.date.accessioned2021-06-23T19:43:49Z
dc.date.available2021-06-23T19:43:49Z
dc.date.issued2016
dc.departmentBAİBÜ, Mudurnu Süreyya Astarcı Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümüen_US
dc.description.abstractThe aim of this study was to evaluate whether the sub-chronic systemic ethanol exposure has direct effect on cavernosal smooth muscle contractions induced by KCl (depolarizing) and phenylephrine (alpha(1)-receptor agonist), and the possible involvement of RhoA/Rho-kinase pathway. Sub-chronic systemic ethanol was applied to mice with inhalation route for 14 days. The blood levels in ethanol-treated mice averaged 121.2 +/- 9.1 mg/dl. KCl (80 mM) and phenylephrine (10 nM-100 mu M) induced sustained contractions in corpus corporal strips from sham-treated mice. Sub-chronic ethanol treatment reduced the contractions to KCl. However, phenylephrine-induced contractions were not affected by ethanol treatment. Rho-kinase inhibitor fasudil (50 mu M) and Y-27632 (50 mu M) inhibited contractions to KCl and phenylephrine in sham-treated mice. Ethanol treatment increased the inhibitory effect of Rho-kinase inhibitors on contractions to phenylephrine. The relaxations induced by fasudil (100 mu M) and Y-27632 (500 mu M) did not change in ethanol treatment group. In ethanol-treated group, the expression of RhoA decreased compared to sham-treated group. Also, ROCK1 expression was reduced by ethanol but not statically significant to sham-treated group; however, the expression of ROCK2 increased in ethanol group. From these findings, it seems that phenylephrine and KCl-induced contractions depends on RhoA/Rho-kinase-mediated Ca2+ sensitization. Also, these results suggest that the ethanol treatment decreased the expression of RhoA, and the inhibitory effect of ethanol on KCl-induced contractions may be due to, at least in part, the inhibition of a RhoA/Rho-kinase in mouse corpus cavernosum.en_US
dc.identifier.doi10.1007/s00210-015-1204-4
dc.identifier.endpage258en_US
dc.identifier.issn0028-1298
dc.identifier.issn1432-1912
dc.identifier.issue3en_US
dc.identifier.pmid26728616en_US
dc.identifier.scopus2-s2.0-84958050587en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage249en_US
dc.identifier.urihttps://doi.org/10.1007/s00210-015-1204-4
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8859
dc.identifier.volume389en_US
dc.identifier.wosWOS:000370053100001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAstarcı, Erhan
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofNaunyn-Schmiedebergs Archives Of Pharmacologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEthanolen_US
dc.subjectKClen_US
dc.subjectPhenylephrineen_US
dc.subjectRhoA/Rho-Kinaseen_US
dc.subjectMouse Corpus Cavernosumen_US
dc.titleThe effect of sub-chronic systemic ethanol treatment on corpus cavernosal smooth muscle contraction: the contribution of RhoA/Rho-kinaseen_US
dc.typeArticleen_US

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