Antimicrobial effectiveness of different irrigation activation techniques on teeth with artificial internal root resorption and contaminated with enterococcus faecalis: A confocal laser scanning,icroscopy analysis

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dc.authorid0000-0002-1773-9114
dc.authorid0000-0002-5331-2206
dc.authorid0000-0003-4252-9538
dc.contributor.authorAkdere, Sevim Koşumcu
dc.contributor.authorAydın, Zeliha Uğur
dc.contributor.authorErdönmez, Demet
dc.date.accessioned2024-09-25T19:57:28Z
dc.date.available2024-09-25T19:57:28Z
dc.date.issued2023
dc.departmentBAİBÜ, Diş Hekimliği Fakültesi, Endodonti Ana Bilim Dalı
dc.description.abstractThis study aimed to compare the antibacterial efficacy of standard needle irrigation (SNI), EDDY, passive ultrasonic irrigation (PUI), photon-induced photoacoustic streaming (PIPS), and shock wave enhanced emission photoacoustic streaming (SWEEPS) activation on the teeth with simulated internal root resorption (IRR) and contaminated with Enterococcus faecalis (E. faecalis) using confocal laser scanning microscopy (CLSM) analyses. A total of 79 human maxillary central incisors with a single canal were selected. The canals were accessed, and then, the roots were split in the bucco-lingual direction. Artificial IRR cavities (depth of 0.8 mm and a diameter of 1.6 mm) were prepared using round burs and 20% nitric acid in the middle region of the root halves. The root halves were reconstructed with cyanoacrylate glue, and the canals were contaminated with a culture of E. faecalis for 30 days. Root canal preparation was performed using the ProTaper Next rotary files up to X5 and 2.5% NaOCl irrigation. Teeth were randomly assigned to five groups according to the irrigation activation method (n = 15): SNI, EDDY, PUI, PIPS, and SWEEPS. The final irrigation procedures were performed using a total of 6 mL of 2.5% NaOCl for each tooth with an activation time of 3 x 30 s. The canals were stained with LIVE/DEAD BacLight dye and analyzed with CLSM to determine the percentages of dead bacteria in the biofilm. Two-way ANOVA and post hoc Tukey tests were used for statistical analysis (P < .05). None of the irrigation activation methods tested provided 100% bacterial elimination. There was no significant difference between the irrigation activation methods tested in terms of the percentage of dead bacteria (P > 0.05). In irrigation activation methods other than PIPS, there was no significant difference in the percentage of dead bacteria between the coronal, middle, and apical regions of the roots (P > 0.05). A higher percentage of dead bacteria was found in the middle region compared to the apical region in the PIPS (P < 0.05). Within the limitations of this study, SII, EDDY, PUI, PIPS, and SWEEPS have a similar antimicrobial effect on the teeth with IRR and contaminated with E. faecalis.en_US
dc.description.sponsorshipBolu Abant Izzet Baysal University, Scientific Research Projects Coordinatorship [2020.06.02.1460]en_US
dc.description.sponsorshipThis study was founded by Bolu Abant Izzet Baysal University, Scientific Research Projects Coordinatorship (Project No: 2020.06.02.1460).en_US
dc.identifier.doi10.1007/s10103-023-03748-8
dc.identifier.issn0268-8921
dc.identifier.issn1435-604X
dc.identifier.issue1en_US
dc.identifier.pmid36943498en_US
dc.identifier.scopus2-s2.0-85150669553en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s10103-023-03748-8
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13443
dc.identifier.volume38en_US
dc.identifier.wosWOS:000954417900001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAkdere, Sevim Koşumcu
dc.institutionauthorid0000-0003-4252-9538
dc.language.isoenen_US
dc.publisherSpringer London Ltden_US
dc.relation.ispartofLasers in Medical Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.snmzYK_20240925en_US
dc.subjectConfocal Laser Scanning Microscopyen_US
dc.subjectEDDYen_US
dc.subjectEnterococcus Faecalisen_US
dc.subjectInternal Root Resorptionen_US
dc.subjectPIPSen_US
dc.subjectSWEEPSen_US
dc.titleAntimicrobial effectiveness of different irrigation activation techniques on teeth with artificial internal root resorption and contaminated with enterococcus faecalis: A confocal laser scanning,icroscopy analysisen_US
dc.typeArticleen_US

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