The electrophysiological and behavioral evaluation of the peptide hemopressin and cannabinoid CB1 receptor agonist and antagonist in pentylenetetrazol model of epilepsy in rats

dc.contributor.authorKaleel, Ali Al
dc.contributor.authorAygün, Hatice
dc.contributor.authorGailani, Lubna Al
dc.contributor.authorKabak, Yonca
dc.contributor.authorİnal, Sinem
dc.contributor.authorAyyıldız, Mustafa
dc.contributor.authorHim, Aydın
dc.date.accessioned2023-08-10T08:30:48Z
dc.date.available2023-08-10T08:30:48Z
dc.date.issued2023en_US
dc.departmentBAİBÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionThis work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK) [project number 215S808]. The funding source had no involvement in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; and in the decision to submit the article for publication.en_US
dc.description.abstractThis study endeavoured to assess the effect of hemopressin (Hp), a nano peptide obtained from the alpha chain of hemoglobin, on chronic epileptic activity and its potential correlation with cannabinoid receptor type 1 (CB1). Male Wistar albino rats (230-260 g) were used. The kindling process was conducted by administering a sub-convulsant dose of pentylenetetrazol (PTZ) (35 mg/kg, i.p) three times a week for a maximum of 10 weeks. Tripolar electrodes and external cannula guides for intracerebroventricular (i.c.v) injections were surgically implanted in the skulls of kindled rats. On the day of the experiment, doses of Hp, AM-251, and ACEA were administered prior to the PTZ injections. Electroencephalography recordings and behavioural observations were conducted simultaneously for 30 min after the PTZ injection. The administration of Hp (0.6 mu g, i.c.v) resulted in a decrease in epileptic activity. The CB1 receptor agonist ACEA (7.5 mu g, i.c.v) showed an anticonvulsant effect, but the CB1 receptor antagonist AM-251 (0.5 mu g, i.c.v) displayed a proconvulsant effect. The co-administration of Hp (0.6 mu g, i.c.v) and ACEA (7.5 mu g, i.c.v) and of Hp (0.6 mu g, i.c.v) and AM-251 (0.5 mu g, i.c.v) produced an anticonvulsant effect. However, when AM-251 was administered prior to Hp, it produced a proconvulsant impact that overrode Hp's intended anticonvulsant effect. Interestingly, the co-administration of Hp (0.03 mu g) + AM-251 (0.125 mu g) unexpectedly exhibited an anticonvulsant effect. Electrophysiological and behavioural evaluations demonstrated the anticonvulsant effect of Hp in the present model, highlighting the possibility that Hp may act as an agonist for the CB1 receptor.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [215S808]en_US
dc.identifier.doi10.1007/s00424-023-02814-y
dc.identifier.endpage730en_US
dc.identifier.issn0031-6768
dc.identifier.issn1432-2013
dc.identifier.pmid37100982en_US
dc.identifier.scopus2-s2.0-85153585361en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage719en_US
dc.identifier.urihttp://dx.doi.org/10.1007/s00424-023-02814-y
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11476
dc.identifier.volume475en_US
dc.identifier.wosWOS:000976802300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorHim, Aydın
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofPflügers Archiv - European Journal of Physiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitak[215S808]
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectACEAen_US
dc.subjectAM-251en_US
dc.subjectCannabinoid Receptoren_US
dc.subjectInduced Epileptiform Activityen_US
dc.subjectEndocannabinoid Systemen_US
dc.subjectInduced Seizureen_US
dc.titleThe electrophysiological and behavioral evaluation of the peptide hemopressin and cannabinoid CB1 receptor agonist and antagonist in pentylenetetrazol model of epilepsy in ratsen_US
dc.typeArticleen_US

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