The association between different molecular weights of hyaluronic acid and CHAD, HIF-1 alpha, COL2A1 expression in chondrocyte cultures

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dc.authorid0000-0001-5590-0637en_US
dc.authorid0000-0002-8084-7855en_US
dc.authorid0000-0001-8171-5929en_US
dc.authorid0000-0003-2003-6337en_US
dc.authorid0000-0002-5412-8355
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorKaplan, Necati
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorAkyuva, Yener
dc.contributor.authorKaya, Yasin Emre
dc.date.accessioned2021-06-23T19:49:46Z
dc.date.available2021-06-23T19:49:46Z
dc.date.issued2018
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractThe aim of the present study was to investigate the effects of three different formulations of hyaluronic acid (HA): Low molecular weight (MW) Sinovial One((R)), medium MW Viscoplus((R)) and high MW Durolane((R)), on chondrocyte proliferation and collagen type II (COL2A1), hypoxia-inducible factor 1 alpha (HIF-1 alpha) and chondroadherin (CHAD) expression in primary chondrocyte cultures. Standard primary chondrocyte cultures were established from osteochondral tissues surgically obtained from 6 patients with gonarthrosis. Cell morphology was evaluated using an inverted light microscope; cell proliferation was determined with a MTT assay and confirmed with acridine orange/propidium iodide staining. Levels of CHAD, COL2A1 and HIF-1 alpha expression were assessed using specific TaqMan gene expression assays. The results demonstrated the positive effect of HA treatment on cell proliferation, which was independent from the MW. COL2A1 expression increased in the medium and high MW HA treated groups. It was observed that HIF-1 alpha expression increased in the high MW treated group alone. CHAD expression increased only in the medium MW HA treated group. Evaluation of gene expression revealed that levels of expression increased as the duration of HA application increased, in the medium and high MW HA treated groups. In terms of increased viability and proliferation, a longer duration of HA application was more effective. Taken together, it may be concluded that the administration of medium and high MW HA may be a successful way of treating diseases affecting chondrocytes in a clinical setting.en_US
dc.identifier.doi10.3892/etm.2018.5943
dc.identifier.endpage4212en_US
dc.identifier.issn1792-0981
dc.identifier.issn1792-1015
dc.identifier.issue5en_US
dc.identifier.pmid29849772en_US
dc.identifier.scopus2-s2.0-85044444457en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage4205en_US
dc.identifier.urihttps://doi.org/10.3892/etm.2018.5943
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9609
dc.identifier.volume15en_US
dc.identifier.wosWOS:000431850300018en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaya, Yasin Emre
dc.language.isoenen_US
dc.publisherSpandidos Publ Ltden_US
dc.relation.ispartofExperimental And Therapeutic Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChondroadherinen_US
dc.subjectCollagen type IIen_US
dc.subjectHypoxia-inducible Factor 1 Alphaen_US
dc.subjectHyaluronic Aciden_US
dc.subjectChondrocytesen_US
dc.titleThe association between different molecular weights of hyaluronic acid and CHAD, HIF-1 alpha, COL2A1 expression in chondrocyte culturesen_US
dc.typeArticleen_US

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