Efficient plant regeneration and callus induction from nodal and hypocotyl explants of goji berry (Lycium barbarum L.) and comparison of phenolic profiles in calli formed under different combinations of plant growth regulators

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dc.authorid0000-0001-5245-6294
dc.contributor.authorKarakaş, Fatma Pehlivan
dc.date.accessioned2021-06-23T19:54:54Z
dc.date.available2021-06-23T19:54:54Z
dc.date.issued2020
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractGoji berry (Lycium barbarum L.) is a fruit that has many beneficial effects (such as antidiabetic, antioxidant, anticancer, antidepressant, and immunomodulatory) on human health because of several dietary constituents such as phenolics, vitamins, carotenoids, and polysaccharides. In order to develop in vitro culture protocols for callus induction and plant regeneration from different explants of Goji plants and to compare the phenolic composition in calli of different origin, various combinations of plant growth regulators (PGRs) were applied. Various types of explants (leaf, petiole, root, hypocotyl, and node) were cultured on MS medium containing 28 different concentrations and combinations of PGRs [thidiazuron (TDZ) and naphthalene acetic acid (NAA), TDZ and indole-3-acetic acid (IAA), benzyl adenine (BA) and NAA, alone TDZ, and alone BA]. The highest mean number of shoots (23.33 +/- 1.86) and percentage of shoot formation (100%) were obtained from nodal explants on medium containing 0.5 mg/l BA alone. The highest mean callus diameter was obtained from hypocotyl explants on medium containing 0.25 mg/l TDZ and 0.1 mg/l IAA (21.40 +/- 0.71 mm). The amounts of selected phenolic substances were significantly different in the callus obtained from different PGRs or combinations, individually. The combination of BA/NAA significantly increased the production and accumulation of chlorogenic acid and caffeic acid. The TDZ/IAA combination, TDZ alone, and TDZ/NAA combination significantly increased vanillic acid and rutin, gallic acid, and quercetin synthesis, respectively. These outcomes indicate that different PGRs lead to the production of different kinds of secondary metabolites and affect/accelerate accumulation in the callus of goji berry. The culture protocol described in this paper could be employed for the development of novel methods for the commercial production of goji secondary metabolites.en_US
dc.identifier.doi10.1016/j.plaphy.2019.11.009
dc.identifier.endpage391en_US
dc.identifier.issn0981-9428
dc.identifier.pmid31790925en_US
dc.identifier.scopus2-s2.0-85075627718en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage384en_US
dc.identifier.urihttps://doi.org/10.1016/j.plaphy.2019.11.009
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10678
dc.identifier.volume146en_US
dc.identifier.wosWOS:000510528300036en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKarakaş, Fatma Pehlivan
dc.language.isoenen_US
dc.publisherElsevier France-Editions Scientifiques Medicales Elsevieren_US
dc.relation.ispartofPlant Physiology And Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCallus Cultureen_US
dc.subjectLC-MS/MS Analysisen_US
dc.subjectPlant Regenerationen_US
dc.subjectPGRsen_US
dc.subjectPhenolicsen_US
dc.subjectSecondary Metabolitesen_US
dc.subjectSuperfruiten_US
dc.titleEfficient plant regeneration and callus induction from nodal and hypocotyl explants of goji berry (Lycium barbarum L.) and comparison of phenolic profiles in calli formed under different combinations of plant growth regulatorsen_US
dc.typeArticleen_US

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