DNA-bioprotective effects of lactic acid bacteria against aflatoxin B-1
Yükleniyor...
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Enviro Research Publishers
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Lactic acid bacteria commonly named as probiotics have a broad range of utilization area in human diet and food industry. Besides these known properties of probiotics, in recent years many researchers have focused on their anti-genotoxic called DNA-bioprotective effects. Human gets exposure with genotoxins such as mycotoxins, polycyclic aromatic hydrocarbons, and n-nitroso-compounds through diet and environmental contaminations. Aflatoxin B-1 is mycotoxin which was reported one of the most potent hepato-carcinogen and its exposure stems from human diet. In this study, we aimed to investigate DNA-bioprotective effect of Lactobacillus plantarum on human colon adenocarcinoma (Caco-2) cells against Aflatoxin B-1 (AFB(1)) with comet assay without metabolic activation. The results showed that DNA-bioprotective effect of L. plantarum did reduce the AFB(1)'s genotoxic effect on colon adenocarcinoma (Caco-2) cells. Positive control (50 iM H2O2 applied) and high dose (>10ppm) AFB(1) applied cells have the same comet tail appearance. Only visual scoring is performed. Besides negative control cells (Only PBS) and Lactobacillus plantarum+AFB(1) mixture showed the same manner with each other; no comet tail detected. These results clearly indicate that L. plantarum is capable of reduce AFB(1) safely without producing any by-products.
Açıklama
Anahtar Kelimeler
Probiotics, Aflatoxin B-1, L. Plantarum, Comet Assay (SCGE)
Kaynak
Current Research In Nutrition And Food Science
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
4
