Preparation of cryogel columns for depletion of hemoglobin from human blood

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dc.authorid0000-0001-8668-8296
dc.authorid0000-0003-3260-1639
dc.authorid0000-0001-8178-0755
dc.authorid0000-0001-7548-5741
dc.authorid0000-0002-4757-0161
dc.contributor.authorDerazshamshir, Ali
dc.contributor.authorBaydemir, Gözde
dc.contributor.authorYılmaz, Fatma
dc.contributor.authorBereli, Nilay
dc.contributor.authorDenizli, Adil
dc.date.accessioned2021-06-23T19:43:36Z
dc.date.available2021-06-23T19:43:36Z
dc.date.issued2016
dc.departmentBAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümüen_US
dc.description.abstractIn this study, we aimed to prepare the metal chelate affinity cryogels for the hemoglobin (Hb) depletion. Poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were selected as base matrix because of their blood compatibility, osmotic, chemical, and mechanical stability. Cryogels are also useful when working with the viscous samples such as blood, because of their interconnected macroporous structure. Iminodiacetic acid (IDA), the chelating agent, was covalently coupled with PHEMA cryogels after activation with the epichlorohydrin and then the Ni(II) ions were chelated to the IDA-bound cryogels. The depletion of the Hb from hemolysate was shown by SDS-PAGE.en_US
dc.identifier.doi10.3109/21691401.2015.1129623
dc.identifier.endpage799en_US
dc.identifier.issn2169-1401
dc.identifier.issn2169-141X
dc.identifier.issue3en_US
dc.identifier.pmid26772759en_US
dc.identifier.scopus2-s2.0-84969969836en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage792en_US
dc.identifier.urihttps://doi.org/10.3109/21691401.2015.1129623
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8812
dc.identifier.volume44en_US
dc.identifier.wosWOS:000376136500005en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorYılmaz, Fatma
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofArtificial Cells Nanomedicine And Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHemoglobin Depletionen_US
dc.subjectIDAen_US
dc.subjectMetal Chelate Affinity Sorbentsen_US
dc.subjectPHEMA Cryogelen_US
dc.titlePreparation of cryogel columns for depletion of hemoglobin from human blooden_US
dc.typeArticleen_US

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