Preparation of cryogel columns for depletion of hemoglobin from human blood
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Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, we aimed to prepare the metal chelate affinity cryogels for the hemoglobin (Hb) depletion. Poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were selected as base matrix because of their blood compatibility, osmotic, chemical, and mechanical stability. Cryogels are also useful when working with the viscous samples such as blood, because of their interconnected macroporous structure. Iminodiacetic acid (IDA), the chelating agent, was covalently coupled with PHEMA cryogels after activation with the epichlorohydrin and then the Ni(II) ions were chelated to the IDA-bound cryogels. The depletion of the Hb from hemolysate was shown by SDS-PAGE.
Açıklama
Anahtar Kelimeler
Hemoglobin Depletion, IDA, Metal Chelate Affinity Sorbents, PHEMA Cryogel
Kaynak
Artificial Cells Nanomedicine And Biotechnology
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
44
Sayı
3
