Comparison of two ELISA methods for detection of antibodies against foot-and-mouth disease virus of cattle breeds in Turkey

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dc.authorid0000-0001-8302-9607en_US
dc.authorid0000-0002-3499-4088
dc.contributor.authorİnce, Ömer Barış
dc.contributor.authorKalkan, Ramazan
dc.contributor.authorÇakır, Serkan
dc.date.accessioned2021-06-23T19:44:19Z
dc.date.available2021-06-23T19:44:19Z
dc.date.issued2016
dc.departmentBAİBÜ, Ziraat Fakültesi, Kanatlı Hayvan Yetiştiriciliği Bölümüen_US
dc.description.abstractThe study was conducted using two ELISA methods-the liquid phase blocking ELISA (LPBE) and solid phase competition ELISA (SPCE) for the detection of foot-and-mouth disease virus (FMDV) serotype A- and O-specific antibodies of different cattle breeds in Turkey. These methods were compared in 426 cattle previously vaccinated with oil-adjuvanted bivalent vaccine as well as in sera from 40 cattle with no history of foot-and-mouth disease infection or vaccination. The results were found that SPCE had a better specificity (serotype A; 100% and serotype O; 97.50%) than LPBE (serotype A 95.00% and serotype O 92.50%). Sensitivity of SPCE had also better values (serotype A; 99.30% and serotype O; 98.59%) than LPBE (serotype A; 97.89% and serotype O; 96.48%). The results of the present study showed that the SPCE method is more reliable than LPBE.en_US
dc.identifier.doi10.12681/jhvms.15621
dc.identifier.endpage38en_US
dc.identifier.issn1792-2720
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84990209764en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage33en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8960
dc.identifier.urihttps://www.webofscience.com/wos/woscc/full-record/WOS:000385013900004
dc.identifier.urihttps://doi.org/10.12681/jhvms.15621
dc.identifier.volume67en_US
dc.identifier.wosWOS:000385013900004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÇakır, Serkan
dc.language.isoenen_US
dc.publisherHellenic Veterinary Medical Socen_US
dc.relation.ispartofJournal Of The Hellenic Veterinary Medical Societyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFoot-and-Mouth Diseaseen_US
dc.subjectLiquid-Phase Blocking ELISAen_US
dc.subjectSolid-Phase Competition ELISAen_US
dc.titleComparison of two ELISA methods for detection of antibodies against foot-and-mouth disease virus of cattle breeds in Turkeyen_US
dc.typeArticleen_US

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