Comparison of two ELISA methods for detection of antibodies against foot-and-mouth disease virus of cattle breeds in Turkey
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Dosyalar
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Hellenic Veterinary Medical Soc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The study was conducted using two ELISA methods-the liquid phase blocking ELISA (LPBE) and solid phase competition ELISA (SPCE) for the detection of foot-and-mouth disease virus (FMDV) serotype A- and O-specific antibodies of different cattle breeds in Turkey. These methods were compared in 426 cattle previously vaccinated with oil-adjuvanted bivalent vaccine as well as in sera from 40 cattle with no history of foot-and-mouth disease infection or vaccination. The results were found that SPCE had a better specificity (serotype A; 100% and serotype O; 97.50%) than LPBE (serotype A 95.00% and serotype O 92.50%). Sensitivity of SPCE had also better values (serotype A; 99.30% and serotype O; 98.59%) than LPBE (serotype A; 97.89% and serotype O; 96.48%). The results of the present study showed that the SPCE method is more reliable than LPBE.
Açıklama
Anahtar Kelimeler
Foot-and-Mouth Disease, Liquid-Phase Blocking ELISA, Solid-Phase Competition ELISA
Kaynak
Journal Of The Hellenic Veterinary Medical Society
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
67
Sayı
1
