Small RNA sequencing based identification of MiRNAs in daphnia magna

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dc.authorid0000-0001-5546-0379en_US
dc.authorid0000-0003-0097-1125en_US
dc.contributor.authorÜnlü, Ercan Selçuk
dc.contributor.authorGordon, Donna M.
dc.contributor.authorTelli, Murat
dc.date.accessioned2021-06-23T19:37:40Z
dc.date.available2021-06-23T19:37:40Z
dc.date.issued2015
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Kimya Bölümüen_US
dc.description.abstractSmall RNA molecules are short, non-coding RNAs identified for their crucial role in post-transcriptional regulation. A well-studied example includes miRNAs (microRNAs) which have been identified in several model organisms including the freshwater flea and planktonic crustacean Daphnia. A model for epigenetic-based studies with an available genome database, the identification of miRNAs and their potential role in regulating Daphnia gene expression has only recently garnered interest. Computational-based work using Daphnia pulex, has indicated the existence of 45 miRNAs, 14 of which have been experimentally verified. To extend this study, we took a sequencing approach towards identifying miRNAs present in a small RNA library isolated from Daphnia magna. Using Perl codes designed for comparative genomic analysis, 815,699 reads were obtained from 4 million raw reads and run against a database file of known miRNA sequences. Using this approach, we have identified 205 putative mature miRNA sequences belonging to 188 distinct miRNA families. Data from this study provides critical information necessary to begin an investigation into a role for these transcripts in the epigenetic regulation of Daphnia magna.en_US
dc.identifier.doi10.1371/journal.pone.0137617
dc.identifier.issn1932-6203
dc.identifier.issue9en_US
dc.identifier.pmid26367422en_US
dc.identifier.scopus2-s2.0-84947425789en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0137617
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8195
dc.identifier.volume10en_US
dc.identifier.wosWOS:000361601100096en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÜnlü, Ercan Selçuk
dc.institutionauthorTelli, Murat
dc.language.isoenen_US
dc.publisherPublic Library Scienceen_US
dc.relation.ispartofPlos Oneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDaphnia Magnaen_US
dc.subjectSmall RNA Sequencing
dc.titleSmall RNA sequencing based identification of MiRNAs in daphnia magnaen_US
dc.typeArticleen_US

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