Patterns of expression of h2s-producing enzyme in human renal cell carcinoma specimens: Potential avenue for future therapeutics

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dc.authorid0000-0002-1454-5672
dc.contributor.authorSöğütdelen, Emrullah
dc.contributor.authorPacoli, Katharine
dc.contributor.authorJuriasingani, Smriti
dc.contributor.authorAkbari, Masoud
dc.contributor.authorGabril, Manal
dc.contributor.authorŞener, Alp
dc.date.accessioned2021-06-23T19:53:57Z
dc.date.available2021-06-23T19:53:57Z
dc.date.issued2020
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractBackground: Renal cell carcinoma (RCC) is the most common cancer of the kidney. The most common histotype is clear-cell (cc) RCC. Hydrogen sulfide (H2S) is an angiogenic and anti-apoptotic gasotransmitter that is elevated under pseudohypoxic conditions. H2S is endogenously produced by three enzymes: Cystathionine gamma-lyase (CSE), cystathionine beta-synthase (CBS), and 3-mercaptopyruvate sulfurtransferase (MPST). Seeing as increased expression of these enzymes has been observed in other human cancer types, this study aimed to quantify H2S-producing enzyme expression in human RCC samples and evaluate whether it correlated with clinical outcomes. Patients and Methods: Eighty-eight human kidney tissue specimens, with healthy and cancerous tissue components, were immunohistochemically stained for CSE, CBS, and MPST. The mean pixel intensity of positively stained areas was quantified. A retrospective analysis was conducted to obtain patient demographics, rates of metastasis/recurrence, and prognostic characteristics. Statistical correlations between enzyme expressions and subsequent patient outcomes were evaluated. Results: There was significantly greater expression of CSE, CBS, and MPST in cc-RCC compared to paired healthy tissue (p<0.0001). The difference in expression of CSE in cancerous versus normal tissue was significantly greater than that for CBS and MPST (p<0.000I and p<0.01, respectively). Enzyme expression patterns in cancerous versus normal tissue did not correlate with nuclear grade, stage, histological type or cancer recurrence/metastasis. Conclusion: To our knowledge, this is the first report of the differential increase in expression of CSE, CBS, and MPST in human RCC. Although these patterns do not appear to correlate with cancer recurrence, metastasis, size or nuclear grade, their differential increase suggests a potential therapeutic target.en_US
dc.identifier.doi10.21873/invivo.12102
dc.identifier.endpage2781en_US
dc.identifier.issn0258-851X
dc.identifier.issn1791-7549
dc.identifier.issue5en_US
dc.identifier.pmid32871814en_US
dc.identifier.scopus2-s2.0-85090181066en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2775en_US
dc.identifier.urihttps://doi.org/10.21873/invivo.12102
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10347
dc.identifier.volume34en_US
dc.identifier.wosWOS:000573472800024en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorSöğütdelen, Emrullah
dc.language.isoenen_US
dc.publisherInt Inst Anticancer Researchen_US
dc.relation.ispartofIn Vivoen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectRenal Cell Carcinomaen_US
dc.subjectHydrogen Sulfideen_US
dc.subjectCystathionine Gamma-lyaseen_US
dc.subjectCSEen_US
dc.subjectCystathionine Beta-synthaseen_US
dc.subjectCBSen_US
dc.subject3-mercaptopyruvate Sulfurtransferaseen_US
dc.subjectMPSTen_US
dc.titlePatterns of expression of h2s-producing enzyme in human renal cell carcinoma specimens: Potential avenue for future therapeuticsen_US
dc.typeArticleen_US

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