Fluctuation in secondary metabolite production and antioxidant defense enzymes in in vitro callus cultures of goat's rue (Galega officinalis) under different abiotic stress treatments

dc.contributor.authorKarakaş, Fatma Pehlivan
dc.contributor.authorBozat, Bihter Gökçe
dc.date.accessioned2021-06-23T19:54:09Z
dc.date.available2021-06-23T19:54:09Z
dc.date.issued2020
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractExogenous abiotic stress treatments are an effective way of accelerating stress tolerance by modulation of accumulation of various secondary metabolites in pharmaceutical, medicinal, and economically important plant species. This research aimed to evaluate the effects of nutrient, chemical, temperature, and ultraviolet (UV) radiation abiotic stress on fluctuations in selected secondary metabolites and the antioxidant defense system ofGalega officinalisL. (goat's rue) callus tissue cultures. Stress treatments were achieved by exposing callus cultures of goat's rue to five different strengths of Murashige and Skoog (MS) medium (2MS, MS, MS/2, MS/4, and MS/8), four different temperatures (4 degrees C, 22 degrees C, 35 degrees C, and 45 degrees C), two chemicals [mercury chloride (HgCl2) and copper sulfate (CuSO4)], and three types of UV radiation (UV-A, UV-B, and UV-C) for three different durations (15, 30, and 60 min). The amounts of selected phenolic secondary metabolites (apigenin,p-coumaric acid, genistein, kaempferol, luteolin, rutin hydrate,trans-ferulic acid, salicylic acid, chlorogenic acid, naringenin, and isorhamnetin) in callus cultures of goat's rue was determined by liquid chromatography-electrospray ionization-multistage/mass spectrometry (LC-ESI-MS/MS). The dominant molecule wasp-coumaric acid in all stress treatments. The highest amounts of luteolin, genistein,p-coumaric, naringenin, apigenin,trans-ferulic acid, salicylic acid, and rutin were determined in the MS/8, MS/8, MS/2, MS/2, MS/4, UV-A (15 min), UV-A (15 min), and 4 degrees C treatments, respectively. Interestingly, the percentage increase (379.01%) in the total selected phenolic molecules was highest in the MS/8 treatment. The superoxide dismutase and catalase antioxidant enzyme activity were highest in the CuSO(4)and MS/4 treatments, respectively. Moreover, the MS/4 treatment resulted in the highest proline content. The total phenolic content was highest after the temperature (4 degrees C) and UV radiation (UV-A 30 min) treatment. The UV-A (15 min) treatment had the strongest antioxidant activity, with the highest total flavonoid content and the lowest concentration inhibiting 50% of DPPH (IC50) value. These outcomes indicated that different abiotic stress factors caused extraordinary variations in antioxidant levels and secondary metabolite accumulation as a way of regulating the plant's defense system. The newly developed protocol in the current study can be used to provide the desired amount of special secondary metabolite production and establish a new valuable herbal medicine to cure many chronic diseases, as well as in the pharmaceutical area and other related areas. Key message Different abiotic stress factors caused extraordinary variations in the antioxidants levels and secondary metabolite accumulation as a way of regulating the plant's defense system.en_US
dc.identifier.doi10.1007/s11240-020-01870-x
dc.identifier.endpage414en_US
dc.identifier.issn0167-6857
dc.identifier.issn1573-5044
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85086771984en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage401en_US
dc.identifier.urihttps://doi.org/10.1007/s11240-020-01870-x
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10438
dc.identifier.volume142en_US
dc.identifier.wosWOS:000542059000001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKarakaş, Fatma Pehlivan
dc.institutionauthorBozat, Bihter Gökçe
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPlant Cell Tissue And Organ Cultureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAbiotic Stressen_US
dc.subjectLC-MSen_US
dc.subjectMS Analysisen_US
dc.subjectPlant Tissue Cultureen_US
dc.subjectPhenolic Accumulationen_US
dc.subjectSecondary Metabolitesen_US
dc.titleFluctuation in secondary metabolite production and antioxidant defense enzymes in in vitro callus cultures of goat's rue (Galega officinalis) under different abiotic stress treatmentsen_US
dc.typeArticleen_US

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