SDF-1 modulates periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs)

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dc.authorid0000-0001-8665-6235en_US
dc.authorid0000-0001-7147-7875en_US
dc.authorid0000-0002-9992-833Xen_US
dc.authorid0000-0003-4791-8946en_US
dc.contributor.authorHakkı, Sema S.
dc.contributor.authorBozkurt, Buket S.
dc.contributor.authorHakkı, Erdoğan Eşref
dc.contributor.authorKaraöz, Erdal
dc.contributor.authorÜnlü, Ali
dc.contributor.authorKayış, Seyit Ali
dc.date.accessioned2023-07-14T11:39:11Z
dc.date.available2023-07-14T11:39:11Z
dc.date.issued2021en_US
dc.departmentBAİBÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümüen_US
dc.descriptionTUBITAK, Grant/Award Number: TUBITAK (SBAG 112S587)en_US
dc.description.abstractBackground/Objectives In this in vitro study, the effects of Stromal cell-derived factor-1 (SDF-1) was evaluated on the periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs) functions. Material and Methods Real-time cell analyzer-single plate (RTCA-SP) was employed for proliferation, and RTCA-dual purpose (DP) was utilized for pdl-MSCs migration potential treated with different SDF-1 concentrations (0, 0.1, 1, 10, 100, 200, and 400 ng/ml). Based on the dose-response findings, 10 ng/ml SDF-1 was used for further mRNA experiments. RNAs isolated at 6 and 24 h were checked using quantitative RT-PCR for mineralized tissue-associated genes including type I collagen (COL I), osteocalcin (OCN), osteopontin (OPN), and runt-related transcription factor 2 (Runx2). cRNA was synthesized for 6 h, and whole-genome array analysis was performed for over 47.000 probes. Data were subjected to quantile normalization before analysis. Results Increased proliferation and migration were observed in pdl-MSCs treated with 0.1, 1, and 10 ng/ml SDF-1. Increased COL I was observed at both time points: 6 and 24 h. While there was no significant change for OCN, OPN, and Runx2 at 6 h, SDF-1 up-regulated OCN and OPN, but down-regulated Runx2 mRNA expressions at 24 h. IL-8 and ESM1 genes were differentially expressed over twofold when the pdl-MSCs were exposed to SDF-1 at whole-genome array analysis. IL-8 induction was confirmed with RT-PCR. Conclusion Findings of this study displayed that SDF-1 modulated pdl-MSCs which were important for periodontal regeneration, inducing migration and proliferation, and regulating extracellular matrix synthesis in favor of the formation of new attachment.en_US
dc.description.sponsorshipTUBITAK [SBAG 112S587]en_US
dc.identifier.citationHakki, S. S., Bozkurt, B. S., Hakki, E. E., Karaoz, E., Unlu, A., & Kayis, S. A. (2021). SDF‐1 modulates periodontal ligament‐Mesenchymal Stem Cells (pdl‐MSCs). Journal of Periodontal Research, 56(4), 774-781.en_US
dc.identifier.doi10.1111/jre.12876
dc.identifier.endpage781en_US
dc.identifier.issn0022-3484
dc.identifier.issn1600-0765
dc.identifier.issue4en_US
dc.identifier.pmid33733508en_US
dc.identifier.scopus2-s2.0-85102633944en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage774en_US
dc.identifier.urihttp://dx.doi.org/10.1111/jre.12876
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11292
dc.identifier.volume56en_US
dc.identifier.wosWOS:000629854200001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKayış, Seyit Ali
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Periodontal Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitak[SBAG 112S587]
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIL-8en_US
dc.subjectmRNA Expressionen_US
dc.subjectPeriodontal Ligament-Mesenchymal Stem Cellsen_US
dc.subjectReal-Time Migrationen_US
dc.subjectReal-Time Proliferationen_US
dc.subjectStromal Cell-Derived Factor-1en_US
dc.titleSDF-1 modulates periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs)en_US
dc.typeArticleen_US

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