Evaluation of biochemical markers during somatic embryogenesis in Silybum marianum L.

dc.authorid0000-0002-6529-2134en_US
dc.authorid0000-0002-6786-3973
dc.contributor.authorAbbasi, Bilal Haider
dc.contributor.authorAli, Huma
dc.contributor.authorYücesan, Buhara
dc.contributor.authorSaeed, Sabahat
dc.contributor.authorRehman, Khalid
dc.date.accessioned2021-06-23T19:43:52Z
dc.date.available2021-06-23T19:43:52Z
dc.date.issued2016
dc.departmentBAİBÜ, Ziraat Fakültesi, Tohum Bilimi ve Teknolojisi Bölümüen_US
dc.description.abstractIn present report effects of explants type, basal media and plant growth regulators (PGRs) were tested for induction of indirect somatic embryogenesis in medicinally important plant Silybum marianum L. Leaf, petiole and root explants were exploited in vitro on B5 (Gamborg), SH (Schenk and Hildebrandt) and MS (Murashige and Skoog) media for induction of embryogenic callus followed by somatic embryogenesis. Highest callus induction frequency (76 +/- 4.8 %) was recorded when petiole explants of in vitro derived plantlets were cultured on B5 medium supplemented with 1.5 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4_D) in combination with 1.5 mg l(-1) Thidiazuron (TDZ). Induction and multiplication of somatic embryos were observed, when the embryogenic calluses were sub-cultured on to B5 medium containing 0.5 mg l(-1) 2,4-D plus 1.5 mg l(-1) TDZ. At this PGRs treatment, 77 % of the cultures responded with 39.1 somatic embryos per callus. Furthermore, MS0 medium was indicated more reponsive for growth and maturation of somatic embryos. Analysis of biochemical markers during various growth phases in somatic embryogenesis revealed that somatic embryos exhibited highest level of total carbohydrate, starch, ascorbic acid and total free amino acids. However, higher protein levels were detected in non-embryogenic callus. Nevertheless, considerable amount of silymarin (4.1 mg g(-1) DW) was detected in somatic embryos than other growth phases. Thus, the present study concluded that biochemical and physiological changes during embryogenesis are influenced by interplay of explants type, basal media and PGRs.en_US
dc.identifier.doi10.1007/s13205-016-0366-1
dc.identifier.issn2190-572X
dc.identifier.issn2190-5738
dc.identifier.pmid28330141en_US
dc.identifier.scopus2-s2.0-85007275374en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1007/s13205-016-0366-1
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8869
dc.identifier.volume6en_US
dc.identifier.wosWOS:000370366600001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorYücesan, Buhara
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartof3 Biotechen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSilybumen_US
dc.subjectExplanten_US
dc.subjectSomatic Embryoen_US
dc.subjectPlant Growth Regulatoren_US
dc.subjectBiochemicalen_US
dc.subjectHPLCen_US
dc.titleEvaluation of biochemical markers during somatic embryogenesis in Silybum marianum L.en_US
dc.typeArticleen_US

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