Transcriptional response and functional target analyses of miRNA and mRNA to planktivorous fish kairomone exposure in Daphnia magna

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dc.authorid0000-0003-0097-1125en_US
dc.authorid0000-0001-5546-0379en_US
dc.contributor.authorTelli, Murat
dc.contributor.authorGordon, Donna M.
dc.contributor.authorÜnlü, Ercan Selçuk
dc.date.accessioned2023-05-25T08:00:48Z
dc.date.available2023-05-25T08:00:48Z
dc.date.issued2021en_US
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.descriptionThis study was supported by Bolu Abant Izzet Baysal University Scientific Research Projects (BAIBU-BAP, Project No: 2017.03.01.1151). We would like to thank the Bolu Abant I.zzet Baysal University and Mississippi State University Department of Biological Sciences for their support.en_US
dc.description.abstractThe discovery of regulatory functions for microRNAs (miRNA) and recently described miRNA profiles for Daphnia (Brachiopoda, Cladocera) make it an ideal system to probe for epigenetic regulation of predator avoidance mechanisms in aquatic ecosystems. In this study we report that exposing D. magna to kairomone released by predatory fish from birth to the first reproduction resulted in the differential expression of the four miRNAs tested: miR-7, miR-34, miR-317, and miR-375. Normalized transcript levels for each miRNA were found to vary across the exposure period with no clear conserved pattern of expression despite functional target analyses by Gene Ontology (GO), Clusters of Orthologous Groups of proteins (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) indicating that predicted miRNA target genes are likely involved in related biological activities. Analysis of mRNA transcripts (Hsp70, Hsp90 (HSP = heat shock protein), actin, AKT-interacting protein (AKT), Glycogen synthase (GYS) and Insulin growth factor receptor (IGFR)) identified in the literature as kairomone-mediated genes, were also carried out. Similar to that obtained for miRNAs, the mRNA relative expression levels showed varying degrees of temporal regulation across the exposure time with the two heat shock transcripts exhibiting elevated levels at early and late time points of exposure while the AKT, GYS, and IGFR transcripts had a general decrease in relative expression during the first 96 hours. Differential mRNA expression data supports the conclusion that kairomone mediated response is regulated by cost of general biological processes and that of survival under long-term kairomone stress. These studies lay the foundation for future work aimed at linking miRNAs and their target transcripts to changes in the signaling events that govern Daphnia response to kairomone specific stress.en_US
dc.description.sponsorshipBolu Abant Izzet Baysal University Scientific Research Projects (BAIBU-BAP) [2017.03.01.1151]; Bolu Abant I.zzet Baysal University; Mississippi State University Department of Biological Sciencesen_US
dc.identifier.citationTelli, M., Gordon, D. M., & Ünlü, E. S. (2021). Transcriptional response and functional target analyses of miRNA and mRNA to planktivorous fish kairomone exposure in Daphnia magna. Fundamental and Applied Limnology, 195(1), 73-84.en_US
dc.identifier.doi10.1127/fal/2021/1386
dc.identifier.endpage84en_US
dc.identifier.issn1863-9135
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85107480318en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage73en_US
dc.identifier.urihttp://dx.doi.org/10.1127/fal/2021/1386
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10954
dc.identifier.volume195en_US
dc.identifier.wosWOS:000653494900006en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorTelli, Murat
dc.institutionauthorÜnlü, Ercan Selçuk
dc.language.isoenen_US
dc.publisherE Schweızerbartsche Verlagsbuchhandlungen_US
dc.relation.ispartofFundamental And Applied Limnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHeat-Shock Proteinsen_US
dc.subjectLife-History Shiftsen_US
dc.subjectFresh-Wateren_US
dc.subjectInducible Defensesen_US
dc.subjectCadmium Toleranceen_US
dc.subjectGene-Expressionen_US
dc.subjectMicrornasen_US
dc.subjectStressen_US
dc.subjectFleaen_US
dc.subjectMechanismsen_US
dc.titleTranscriptional response and functional target analyses of miRNA and mRNA to planktivorous fish kairomone exposure in Daphnia magnaen_US
dc.typeArticleen_US

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