Transcriptional response and functional target analyses of miRNA and mRNA to planktivorous fish kairomone exposure in Daphnia magna
Küçük Resim Yok
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
E Schweızerbartsche Verlagsbuchhandlung
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The discovery of regulatory functions for microRNAs (miRNA) and recently described miRNA profiles for Daphnia (Brachiopoda, Cladocera) make it an ideal system to probe for epigenetic regulation of predator avoidance mechanisms in aquatic ecosystems. In this study we report that exposing D. magna to kairomone released by predatory fish from birth to the first reproduction resulted in the differential expression of the four miRNAs tested: miR-7, miR-34, miR-317, and miR-375. Normalized transcript levels for each miRNA were found to vary across the exposure period with no clear conserved pattern of expression despite functional target analyses by Gene Ontology (GO), Clusters of Orthologous Groups of proteins (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) indicating that predicted miRNA target genes are likely involved in related biological activities. Analysis of mRNA transcripts (Hsp70, Hsp90 (HSP = heat shock protein), actin, AKT-interacting protein (AKT), Glycogen synthase (GYS) and Insulin growth factor receptor (IGFR)) identified in the literature as kairomone-mediated genes, were also carried out. Similar to that obtained for miRNAs, the mRNA relative expression levels showed varying degrees of temporal regulation across the exposure time with the two heat shock transcripts exhibiting elevated levels at early and late time points of exposure while the AKT, GYS, and IGFR transcripts had a general decrease in relative expression during the first 96 hours. Differential mRNA expression data supports the conclusion that kairomone mediated response is regulated by cost of general biological processes and that of survival under long-term kairomone stress. These studies lay the foundation for future work aimed at linking miRNAs and their target transcripts to changes in the signaling events that govern Daphnia response to kairomone specific stress.
Açıklama
This study was supported by Bolu Abant Izzet Baysal University Scientific Research Projects (BAIBU-BAP, Project No: 2017.03.01.1151). We would like to thank the Bolu Abant I.zzet Baysal University and Mississippi State University Department of Biological Sciences for their support.
Anahtar Kelimeler
Heat-Shock Proteins, Life-History Shifts, Fresh-Water, Inducible Defenses, Cadmium Tolerance, Gene-Expression, Micrornas, Stress, Flea, Mechanisms
Kaynak
Fundamental And Applied Limnology
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
195
Sayı
1
Künye
Telli, M., Gordon, D. M., & Ünlü, E. S. (2021). Transcriptional response and functional target analyses of miRNA and mRNA to planktivorous fish kairomone exposure in Daphnia magna. Fundamental and Applied Limnology, 195(1), 73-84.
