Effects of moderate high temperature and UV-B on accumulation of withanolides and relative expression of the squalene synthase gene in Physalis peruviana

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dc.authorid0000-0001-5245-6294en_US
dc.authorid0000-0003-0060-259Xen_US
dc.authorid0000-0001-5546-0379
dc.authorid0000-0003-0097-1125
dc.contributor.authorŞahin, Günce
dc.contributor.authorTelli, Murat
dc.contributor.authorÜnlü, Ercan Selçuk
dc.contributor.authorPehlivan Karakaş, Fatma
dc.date.accessioned2021-06-23T19:54:44Z
dc.date.available2021-06-23T19:54:44Z
dc.date.issued2020
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractPhysalis peruviana L. (Cape gooseberry) is a source for a variety of phytocompounds such as withanolides, withanone, withaferin A, and withanolide A. These withanolides are high-value drug candidates due to their various pharmacological properties. To meet the increasing demands for these compounds, plant cell technology offers a reliable alternative. Exogenous addition of elicitors is considered the most effective strategy for enhanced production of secondary metabolites. In this study, we investigated changes in withanolide accumulation and characterized the gene expression level changes of squalene synthase enzyme in P. peruviana shoot cultures exposed to mild nonlethal heat stress (45 degrees C for 2 and 5 h) and UV-B radiation (313 nm for 15 min and 3 h). We demonstrated significant changes in withanolide content with 7.86- and 12.5-fold increases for 2- and 5-hmild high-temperature exposure times, respectively. Exposure to UV-B also changed the withanolide content by 7.22- and 7-fold increases for 15 min and 3 h exposure times, respectively. The relative expression level of squalene synthase gene showed consistent results with1.80- and 10.13-fold increases in withanolide for 2- and 5-h mild high-temperature exposure times, and 1.34- and 2.01-fold increases with 15 min and 3 h UV-B exposure times, respectively.en_US
dc.identifier.doi10.3906/biy-2002-69
dc.identifier.endpage303en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue5en_US
dc.identifier.pmid33110367en_US
dc.identifier.scopus2-s2.0-85092463840en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage295en_US
dc.identifier.trdizinid438467en_US
dc.identifier.urihttps://doi.org/10.3906/biy-2002-69
dc.identifier.urihttps://hdl.handle.net/20.500.12491/10631
dc.identifier.volume44en_US
dc.identifier.wosWOS:000582626200008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorŞahin, Günce
dc.institutionauthorTelli, Murat
dc.institutionauthorÜnlü, Ercan Selçuk
dc.institutionauthorPehlivan Karakaş, Fatma
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCape Gooseberryen_US
dc.subjectWithanolidesen_US
dc.subjectSqualene Synthaseen_US
dc.subjectHPLCen_US
dc.subjectqPCR1en_US
dc.titleEffects of moderate high temperature and UV-B on accumulation of withanolides and relative expression of the squalene synthase gene in Physalis peruvianaen_US
dc.typeArticleen_US

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