Determination of melatonin hormone and neurotransmitters in cerebrospinal fluid: method validation and uncertainty calculations

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dc.authorid0000-0003-4632-3457en_US
dc.authorid0000-0002-0107-0110
dc.contributor.authorÇetin, Özge Yetgin
dc.contributor.authorKaradeniz, Hatice
dc.contributor.authorKarakaş, Alper
dc.contributor.authorKarakaş, Serpil Yenisoy
dc.date.accessioned2021-06-23T19:49:40Z
dc.date.available2021-06-23T19:49:40Z
dc.date.issued2018
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Kimya Bölümüen_US
dc.description.abstractThe uncertainty of measurement for well-known neurotransmitters like serotonin, noradrenalin and gamma-aminobutyric acid (GABA), glutamate and melatonin hormone with high performance liquid chromatography-fluorescence detection was calculated after the method validation. Two methods were developed for the determination of the neurotransmitters. A derivatization step was performed for the determination of GABA and glutamate. Sensitivity, method detection limit, limit of quantification, linearity, recovery, interday and intraday precision values were calculated. Low detection limit values were obtained especially for the determination of GABA and glutamate. Then, bottom-up approach was used to calculate measurement uncertainty. The critical stages of the method were evaluated. The major sources of the uncertainty budget were calibration curves, stock solution and recovery. The calculated percentage relative uncertainty values for the compounds changed between 10.1 and 16.7.en_US
dc.identifier.doi10.1134/S1061934818060114
dc.identifier.endpage625en_US
dc.identifier.issn1061-9348
dc.identifier.issn1608-3199
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85048222287en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage613en_US
dc.identifier.urihttps://doi.org/10.1134/S1061934818060114
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9583
dc.identifier.volume73en_US
dc.identifier.wosWOS:000434474800012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorÇetin, Özge Yetgin
dc.institutionauthorKaradeniz, Hatice
dc.institutionauthorKarakaş, Alper
dc.institutionauthorKarakaş, Serpil Yenisoy
dc.language.isoenen_US
dc.publisherPleiades Publishing Incen_US
dc.relation.ispartofJournal Of Analytical Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMethod Validationen_US
dc.subjectUncertaintyen_US
dc.subjectHPLC-FLDen_US
dc.subjectNoradrenalineen_US
dc.subjectSerotoninen_US
dc.subjectMelatoninen_US
dc.subjectGABAen_US
dc.subjectGlutamateen_US
dc.titleDetermination of melatonin hormone and neurotransmitters in cerebrospinal fluid: method validation and uncertainty calculationsen_US
dc.typeArticleen_US

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