An in vitro and in vivo investigation of the cytotoxic effects of caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester and bortezomib in multiple myeloma cells

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dc.authorid0000-0002-8966-1723
dc.contributor.authorAltaylı, Ertan
dc.contributor.authorKoru, Özgür
dc.contributor.authorÖngörü, Önder
dc.contributor.authorİde, Tayfun
dc.contributor.authorAçıkel, Cengizhan
dc.contributor.authorAstarcı, Erhan
dc.date.accessioned2021-06-23T18:40:27Z
dc.date.available2021-06-23T18:40:27Z
dc.date.issued2015
dc.departmentBAİBÜ, Mudurnu Süreyya Astarcı Meslek Yüksekokulu, Bitkisel ve Hayvansal Üretim Bölümüen_US
dc.description.abstractBackground/aim: In this study, the in vitro and in vivo effectiveness of caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester (CAPE) in combination with bortezomib, a proteasome inhibitor, was explored in multiple myeloma (MM) cells. Materials and methods: The cytotoxic effects of CAPE and bortezomib were determined by XTT cell proliferation assay. Apoptosis levels were analyzed with annexin V-fluorescein isothiocyanate, nuclear factor kappa beta (NF-κB) was analyzed with electrophoretic mobility-shift assay, and interleukin (IL)-6 levels were analyzed with enzyme-linked immunosorbent assay to evaluate CAPE’s mechanism of action. To investigate the in vivo effectiveness of CAPE and bortezomib, an experimental plasmacytoma model was induced in BALB/c mice. Results: Increasing concentrations of CAPE and bortezomib decreased the proliferation of ARH-77 cells in a dose-dependent manner. With doses of CAPE IC50, a significant increase in apoptosis and a significant decrease in IL-6 levels were detected. The NF-κB DNA binding activity decreased compared to the basal ARH-77 level. The administration of CAPE alone or in combination with bortezomib increased the rate of survival compared to the control group. Conclusion: We think that our study, which is the first to demonstrate the in vitro and in vivo effectiveness of the combined use of CAPE and bortezomib, will be a pioneer for future human applications of CAPE in MM.en_US
dc.identifier.endpage46en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue1en_US
dc.identifier.pmid25790528en_US
dc.identifier.scopus2-s2.0-84920059428en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage38en_US
dc.identifier.trdizinid211804en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWpFeE9EQTBOQT09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/3110
dc.identifier.volume45en_US
dc.identifier.wosWOS:000347840000007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorAstarcı, Erhan
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCaffeic Acid Phenethyl Esteren_US
dc.subjectBortezomib
dc.subjectMultiple Myeloma
dc.subjectİn Vitro Cytotoxicity
dc.subjectIn Vivo Study
dc.titleAn in vitro and in vivo investigation of the cytotoxic effects of caffeic acid (3,4-dihydroxycinnamic acid) phenethyl ester and bortezomib in multiple myeloma cellsen_US
dc.typeArticleen_US

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