SYBR green dye-based probe-free SNP genotyping : Introduction of T-Plex real-time PCR assay

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dc.authorid0000-0003-2185-3259en_US
dc.contributor.authorBarış, İbrahim
dc.contributor.authorEtlik, Özdal
dc.contributor.authorKöksal, Vedat
dc.contributor.authorOcak, Zeynep
dc.contributor.authorBarış, Saniye Tuğba
dc.date.accessioned2021-06-23T19:34:12Z
dc.date.available2021-06-23T19:34:12Z
dc.date.issued2013
dc.departmentBAİBÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractSingle-nucleotide polymorphism (SNP) genotyping is widely used in genetic association studies to characterize genetic factors underlying inherited traits. Despite many recent advances in high-throughput SNP genotyping, inexpensive and flexible methods with reasonable throughput levels are still needed. Real-time PCR methods for discovering and genotyping SNPs are becoming increasingly important in various fields of biology. In this study, we introduce a new, single-tube strategy that combines the tetra-primer ARMS PCR assay, SYBR Green I-based real-time PCR, and melting-point analysis with primer design strategies to detect the SNP of interest. This assay, T-Plex real-time PCR, is based on the T. discrimination of the amplified allele-specific amplicons in a single tube. The specificity, sensitivity, and robustness of the assay were evaluated for common mutations in the FV, PII, MTHFR, and FGFR3 genes. We believe that T-Plex real-time PCR would be a useful alternative for either individual genotyping requests or large epidemiological studies. (C) 2013 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.ab.2013.07.007
dc.identifier.endpage231en_US
dc.identifier.issn0003-2697
dc.identifier.issue2en_US
dc.identifier.pmid23872005en_US
dc.identifier.scopus2-s2.0-84884928258en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage225en_US
dc.identifier.urihttps://doi.org/10.1016/j.ab.2013.07.007
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7418
dc.identifier.volume441en_US
dc.identifier.wosWOS:000324605400022en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorOcak, Zeynep
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofAnalytical Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTetra-primer ARMS PCRen_US
dc.subjectSNPen_US
dc.subjectFVLen_US
dc.subjectMTHFRen_US
dc.subjectSYBR Greenen_US
dc.subjectT-Plex Real-Time PCRen_US
dc.titleSYBR green dye-based probe-free SNP genotyping : Introduction of T-Plex real-time PCR assayen_US
dc.typeArticleen_US

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