In vitro regeneration and cardenolide determination of an endemic foxglove, Digitalis cariensis (Aegean Foxglove)

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dc.authorid0000-0001-6262-2866en_US
dc.authorid0000-0002-7631-823Xen_US
dc.authorid0000-0002-6786-3973
dc.authorid0000-0002-5647-7559
dc.contributor.authorMohammed, Aliyu
dc.contributor.authorYücesan, Buhara
dc.contributor.authorDemir-Ordu, Öznur
dc.contributor.authorCihangir, Cansu
dc.contributor.authorEker, İsmail
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:41:59Z
dc.date.available2021-06-23T19:41:59Z
dc.date.issued2015
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractThe genus Digitalis L. is one of the most pharmacologically important plant genera because many Digitalis species produce cardenolides, which are commonly used for cardiac insufficiencies. In this study, different pretreatments were employed to increase germination of Digitalis cariensis seeds, which germinate poorly because of extensive dormancy. Seed germination was improved when seeds were pretreated by scarification followed by soaking in sterile distilled water overnight. In addition, the regeneration capacity of four different types of explants (cotyledonary leaf, root, hypocotyl, or flamingo-bill-type [FBT]) was tested on Linsmaier and Skoog (LS), Murashige and Skoog (MS) and Gamborg's B5 (B5) medium containing no plant growth regulators or supplemented with 0.5 mg L-1 thidiazuron (TDZ) alone or in combination with 0.25 mg L-1 indole-3-acetic acid (IAA). Among the explants tested, only FBT explants regenerated. The highest mean number of shoots was produced on LS medium supplemented with a combination of 0.5 mg L-1 TDZ and 0.25 mg L-1 IAA, producing 3.9 shoots per FBT explant after 4 wk of incubation. Regenerated shoots were rooted on LS medium containing different concentrations of IAA or indole-3-butyric acid (IBA) (0.1, 0.5, or 1.0 mg L-1). The greatest number of roots developed on medium supplemented with 1.0 mg L-1 IAA, which produced 5.6 roots per shoot after 3 wk of culture. Cardenolides were profiled by HPLC analysis from basal leaves of D. cariensis plants from natural populations and from in vitro-derived plantlets. There was no significant difference in lanatoside contents (A, B, and C) between the two sources. Digoxin and digitoxin were not detected in either source.en_US
dc.identifier.doi10.1007/s11627-015-9697-6
dc.identifier.endpage444en_US
dc.identifier.issn1054-5476
dc.identifier.issn1475-2689
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84939253775en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage438en_US
dc.identifier.urihttps://doi.org/10.1007/s11627-015-9697-6
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8272
dc.identifier.volume51en_US
dc.identifier.wosWOS:000359929900007en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorMohammed, Aliyu
dc.institutionauthorYücesan, Buhara
dc.institutionauthorDemir-Ordu, Öznur
dc.institutionauthorCihangir, Cansu
dc.institutionauthorEker, İsmail
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofIn Vitro Cellular & Developmental Biology-Planten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCardenolidesen_US
dc.subjectDigitalis cariensisen_US
dc.subjectFlamingo-bill-type explanten_US
dc.subjectIn vitro regenerationen_US
dc.titleIn vitro regeneration and cardenolide determination of an endemic foxglove, Digitalis cariensis (Aegean Foxglove)en_US
dc.typeArticleen_US

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