Evaluating the effects of subnormothermic perfusion with AP39 in a novel blood-free model of ex vivo kidney preservation and reperfusio

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dc.authorid0000-0002-5455-1495en_US
dc.authorid0000-0002-1454-5672en_US
dc.contributor.authorJuriasingani, Smriti
dc.contributor.authorJackson, Ashley
dc.contributor.authorZhang, Max Yulin
dc.contributor.authorRuthirakanthan, Aushanth
dc.contributor.authorDugbartey, George J.
dc.contributor.authorSöğütdelen, Emrullah
dc.date.accessioned2023-07-18T06:12:23Z
dc.date.available2023-07-18T06:12:23Z
dc.date.issued2021en_US
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.descriptionThis research was supported by Physicians Services Incorporated (PSI) Foundation (Grant #18-17) and the Kidney Foundation of Canada (Grant #180015).en_US
dc.description.abstractThe use of blood for normothermic and subnormothermic kidney preservation hinders the translation of these approaches and promising therapeutics. This study evaluates whether adding hydrogen sulfide donor AP39 to Hemopure, a blood substitute, during subnormothermic perfusion improves kidney outcomes. After 30 min of renal pedicle clamping, porcine kidneys were treated to 4 h of static cold storage (SCS-4 degrees C) or subnormothermic perfusion at 21 degrees C with Hemopure (H-21 degrees C), Hemopure + 200 nM AP39 (H200nM-21 degrees C) or Hemopure + 1 mu M AP39 (H1 mu M-21 degrees C). Then, kidneys were reperfused with Hemopure at 37 degrees C for 4 h with metabolic support. Perfusate composition, tissue oxygenation, urinalysis and histopathology were analyzed. During preservation, the H200nM-21 degrees C group exhibited significantly higher urine output than the other groups and significantly higher tissue oxygenation than the H1 mu M-21 degrees C group at 1 h and 2h. During reperfusion, the H200nM-21 degrees C group exhibited significantly higher urine output and lower urine protein than the other groups. Additionally, the H200nM-21 degrees C group exhibited higher perfusate pO(2) levels than the other groups and significantly lower apoptotic injury than the H-21 degrees C and the H1 mu M-21 degrees C groups. Thus, subnormothermic perfusion at 21 degrees C with Hemopure + 200 nM AP39 improves renal outcomes. Additionally, our novel blood-free model of ex vivo kidney preservation and reperfusion could be useful for studying other therapeutics.en_US
dc.description.sponsorshipPhysicians Services Incorporated (PSI) Foundation [18-17]; Kidney Foundation of Canada [180015]en_US
dc.identifier.citationJuriasingani, S., Jackson, A., Zhang, M. Y., Ruthirakanthan, A., Dugbartey, G. J., Sogutdelen, E., ... & Sener, A. (2021). Evaluating the effects of subnormothermic perfusion with AP39 in a novel blood-free model of ex vivo kidney preservation and reperfusion. International Journal of Molecular Sciences, 22(13), 7180.en_US
dc.identifier.doi10.3390/ijms22137180
dc.identifier.endpage16en_US
dc.identifier.issn1422-0067
dc.identifier.issue13en_US
dc.identifier.pmid34281230en_US
dc.identifier.scopus2-s2.0-85108946995en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttp://dx.doi.org/10.3390/ijms22137180
dc.identifier.urihttps://hdl.handle.net/20.500.12491/11304
dc.identifier.volume22en_US
dc.identifier.wosWOS:000670999000001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorSöğütdelen, Emrullah
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofİnternational Journal of Molecular Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectKidney Preservationen_US
dc.subjectKidney Transplantationen_US
dc.subjectHydrogen Sulfide (H2S)en_US
dc.subjectNormothermic Machine Perfusionen_US
dc.subjectCold Ischemiaen_US
dc.subjectDonationen_US
dc.titleEvaluating the effects of subnormothermic perfusion with AP39 in a novel blood-free model of ex vivo kidney preservation and reperfusioen_US
dc.typeArticleen_US

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