Analysis of genetic diversity among common bean germplasm by start codon targeted (SCoT) markers
Yükleniyor...
Dosyalar
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background Breeding strategies to improve modern varieties having high yield, high nutritional value and resistance to biotic and abiotic stress, etc. is very important to make up for the food deficiencies. Molecular studies as a tool in breeding programs for the characterization of germplasm have been performed with several DNA marker systems. Materials and methods In the present study, the genetic diversity of 53 common bean landraces and 22 registered varieties from Turkey, and 12 genotypes from USDA was investigated using start codon targeted (SCoT) markers for the first time worldwide. The 8 primers having stronger and more polymorphic bands were used for PCR amplification. Results The mean polymorphic band of all primers was found as 13.13. The average of polymorphic information content and resolving power values was 0.34 and 7.55, respectively. Analysis of molecular variance (AMOVA) explored the existence of higher genetic diversity within populations accounting for 92% compared to among populations variations. According to cluster analysis (UPGMA) and genetic structure based on SCoT data, accessions were separated into Andean (PopA) and Mesoamerican PopB) gene pools. Moreover, accessions were mostly placed in the same groups/subgroups according to their geographical origin. Conclusions A high level of genetic diversity was observed between the investigated accessions in this work. The findings will help to plant breeders to characterize common bean accessions.
Açıklama
Scientific and Technological Research Council of Turkey (TUBITAK)(Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)); USDA-ARS Germplasm Resources Information Network (GRIN), Research Institutes of the Republic of Turkey Ministry of Agriculture and Forestry; Bolu Abant Izzet Baysal University Scientific Research Projects Coordination Unit
Anahtar Kelimeler
Genetic Differentiation, Genetic Diversity, Genetic Resources, Phaseolus Breeding, Phaseolus Vulgaris, Population Structure
Kaynak
Molecular Biology Reports
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
49
Sayı
5
Künye
Yeken, M. Z., Emiralioğlu, O., Çiftçi, V., Bayraktar, H., Palacioğlu, G., & Özer, G. (2022). Analysis of genetic diversity among common bean germplasm by start codon targeted (SCoT) markers. Molecular Biology Reports, 49(5), 3839-3847.
