Molecular weight distribution analysis by ultracentrifugation : adaptation of a new approach for mucins

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dc.authorid0000-0003-4007-9609en_US
dc.authorid0000-0002-0335-2162en_US
dc.authorid0000-0002-7607-6808en_US
dc.contributor.authorGillis, Richard B.
dc.contributor.authorAdams, Gary G.
dc.contributor.authorWolf, Bettina
dc.contributor.authorBerry, Monica
dc.contributor.authorBesong, Tabot M. D.
dc.contributor.authorKök, Şamil Mehmet
dc.date.accessioned2021-06-23T19:34:47Z
dc.date.available2021-06-23T19:34:47Z
dc.date.issued2013
dc.departmentBAİBÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.description2nd International Polysaccharide Conference on Polysaccharides as Source of Advanced and Sustainable Products (EPNOE) -- AUG 29-SEP 02, 2011 -- Wageningen, NETHERLANDSen_US
dc.description.abstractMucins are the key macromolecular component of mucus, nature's natural lubricant, and one of the most important physical properties is their molecular weight distribution. A new approach for polydisperse polymers was recently published based on sedimentation velocity in the analytical ultracentrifuge and converts a distribution of sedimentation coefficient g(s) vs. s plot into a distribution of molecular weight utilising the power-law or scaling relationship between the sedimentation coefficient and molecular weight, s = kappa(s) M-w(b) where s is the sedimentation coefficient, M-w, is the weight average molecular weight and kappa(s) and bare characteristic coefficients related to conformation. We investigate the possibility of using a large database of previously published values of s an M to define kappa(s) and b for both aqueous solution and aqueous solution supplemented by 6 M guanidine hydrochloride (a solvent which helps to minimise sample degradation). These values are then applied to a study of the molecular weight distributions of preparations of human gastric mucin in the different solvents and at different stages of purification. (C) 2012 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipWageningen Univ & Res Ctr, European Polysaccharide Network Excellenceen_US
dc.identifier.doi10.1016/j.carbpol.2012.05.018
dc.identifier.endpage183en_US
dc.identifier.issn0144-8617
dc.identifier.issn1879-1344
dc.identifier.issue1en_US
dc.identifier.pmid23465917en_US
dc.identifier.scopus2-s2.0-84874648515en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage178en_US
dc.identifier.urihttps://doi.org/10.1016/j.carbpol.2012.05.018
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7622
dc.identifier.volume93en_US
dc.identifier.wosWOS:000316512900025en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKök, Şamil Mehmet
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofCarbohydrate Polymersen_US
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMucinsen_US
dc.subjectExtended Fujita Approachen_US
dc.subjectColumn and Membrane Freeen_US
dc.titleMolecular weight distribution analysis by ultracentrifugation : adaptation of a new approach for mucinsen_US
dc.typeConference Objecten_US

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