Molecular weight distribution analysis by ultracentrifugation : adaptation of a new approach for mucins
Yükleniyor...
Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Mucins are the key macromolecular component of mucus, nature's natural lubricant, and one of the most important physical properties is their molecular weight distribution. A new approach for polydisperse polymers was recently published based on sedimentation velocity in the analytical ultracentrifuge and converts a distribution of sedimentation coefficient g(s) vs. s plot into a distribution of molecular weight utilising the power-law or scaling relationship between the sedimentation coefficient and molecular weight, s = kappa(s) M-w(b) where s is the sedimentation coefficient, M-w, is the weight average molecular weight and kappa(s) and bare characteristic coefficients related to conformation. We investigate the possibility of using a large database of previously published values of s an M to define kappa(s) and b for both aqueous solution and aqueous solution supplemented by 6 M guanidine hydrochloride (a solvent which helps to minimise sample degradation). These values are then applied to a study of the molecular weight distributions of preparations of human gastric mucin in the different solvents and at different stages of purification. (C) 2012 Elsevier Ltd. All rights reserved.
Açıklama
2nd International Polysaccharide Conference on Polysaccharides as Source of Advanced and Sustainable Products (EPNOE) -- AUG 29-SEP 02, 2011 -- Wageningen, NETHERLANDS
Anahtar Kelimeler
Mucins, Extended Fujita Approach, Column and Membrane Free
Kaynak
Carbohydrate Polymers
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
93
Sayı
1
