Effect of metformin on the human T98G glioblastoma multiforme cell line

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dc.authorid0000-0002-5650-1280
dc.authorid0000-0002-3060-1507
dc.authorid0000-0003-0290-5498
dc.contributor.authorÜçbek, Ali
dc.contributor.authorÖzünal, Zeynep Güneş
dc.contributor.authorUzun, Özge
dc.contributor.authorGepdiremen, Akçahan
dc.date.accessioned2021-06-23T19:35:59Z
dc.date.available2021-06-23T19:35:59Z
dc.date.issued2014
dc.departmentBAİBÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümüen_US
dc.description.abstractMetformin is a guanidine derivative found in Galega officinalis that is commonly used to treat diabetes mellitus. The mechanism of action of metformin involves regulation of the adenosine monophosphate-activated protein kinase/mammalian target of rapamycin signaling pathway, which is implicated in the control of protein synthesis and cell proliferation. This led to the hypothesis that metformin reduces the risk of cancer and slows tumor growth. Thus, in the present study, the effectiveness of metformin as an antiglioma agent was evaluated using the human T98G glioblastoma multiforme cell line. The viability of the T98G cells was assessed using a 3-(4,5-dimethylthiazol-2-yL)-2,5-diphenyltetrazolium bromide assay. Apoptosis was monitored by measuring caspase-3 levels, as well as by terminal deoxynucleotidyl transferase dUTP nick end labeling and staining with acridine orange and ethidium bromide. The results demonstrate that metformin reduced cell viability and caused apoptotic morphological changes in the T98G cells. Furthermore, the caspase-3 levels in the metformin-treated T98G cells were higher than those in the control cells. Metformin induced apoptosis in the T98G cell line in a concentration-dependent manner. Metformin may provide an important contribution to the treatment of glioblastoma multiforme.en_US
dc.identifier.doi10.3892/etm.2014.1597
dc.identifier.endpage1290en_US
dc.identifier.issn1792-0981
dc.identifier.issn1792-1015
dc.identifier.issue5en_US
dc.identifier.pmid24940426en_US
dc.identifier.scopus2-s2.0-84896838154en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage1285en_US
dc.identifier.urihttps://doi.org/10.3892/etm.2014.1597
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7908
dc.identifier.volume7en_US
dc.identifier.wosWOS:000337557700040en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorÜçbek, Ali
dc.institutionauthorÖzünal, Zeynep Güneş
dc.institutionauthorUzun, Özge
dc.institutionauthorGepdiremen, Akçahan
dc.language.isoenen_US
dc.publisherSpandidos Publ Ltden_US
dc.relation.ispartofExperimental And Therapeutic Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMetforminen_US
dc.subjectT98Gen_US
dc.subjectGlioblastoma Cell Lineen_US
dc.subjectApoptosisen_US
dc.titleEffect of metformin on the human T98G glioblastoma multiforme cell lineen_US
dc.typeArticleen_US

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