Attenuation of ischemia/reperfusion injury by n-acetylcysteine in a rat hind limb model
Yükleniyor...
Dosyalar
Tarih
2003
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press Inc Elsevier Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Background. Ischemia/reperfusion is a complex set of events with severe pathologic consequences. Reperfusion initiates both the local and systemic damage in part through rapid oxygen generation. N-acetylcysteine (NAC) is a scavenger of free radical species, inhibits neutrophil accumulation, acts as a vasodilator and also improves microcirculation. In present study, we examined the protective effect of NAC in a rat hind limb ischemia/reperfusion model. Dimethyl-sulfoxide (DMSO), a well-known antioxidant was also tested for comparison. Materials and methods. Ischemia was induced for 4 h by vascular clamping and followed by 1 h of reperfusion. Muscle injury was evaluated in 3 groups as a saline group (control), DMSO group, and NAC group. Plasma levels of creatine kinase, lactate dehydrogenase, thiobarbituric acid reactive substances (TBARS), and blood HCO3, as well as muscle tissue TBARS, were measured at the end of reperfusion. Muscle tissue samples were taken for histological evaluation. Results. DMSO and NAC group showed significant amelioration of plasma CPK (P < 0.05, P < 0.05), plasma TBARS (P < 0.05, P < 0.05), and muscle tissue TBARS (P < 0.05, P < 0.05) compared with the control group. Similarly, neutrophil infiltration in DMSO and NAC groups were significantly less prominent than the control group (P < 0.01, P < 0.01). Conclusions. These results show that NAC improved effectively ischemia reperfusion injury in a rat hind limb model.
Açıklama
Anahtar Kelimeler
N-acetylcysteine, Ischemia/Reperfusion Injury, Dimethyl Sulfoxide, Thiobarbituric Acid Reactive Substances, Histological Examination
Kaynak
Journal Of Surgical Research
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
111
Sayı
2
