Investigation of virulence factors and determination of antifungal susceptibilities of candida strains isolated at Suleyman Demirel University Hospital
Yükleniyor...
Dosyalar
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Carbone Editore
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective: The aim of this study was to evaluate the virulence factors and antifungal susceptibilities of Candida strains, which were isolated from various clinical specimens. Methods: One-hundred Candida strains were identified. Bovine serum albumin agar was used to investigate proteinase production and egg yolk agar was used to test phospholipase production. Modified tube adherence method was used for investigation of slime activity. The antifungal susceptibilities of the strains for amphotericin B, fluconazole, voriconazole and caspofungin were evaluated by using reference broth microdilution method. Results: Forty-eight percent (n=48) of the Candida strains were identified as C. albicans which represented the most common species. Both proteinase and phospholipase production rates were significantly higher among C. albicans isolates (79%; 66%) than non albicans Candida strains (11.5%; 0%) (r=33.22, p<0.001 and r=206.8, p<0.001 repectively). On the other hand, slime activity was significantly higher among non-albicans strains (30%) when compered with C. albicans (0%) (r=0.228, p<0.001). MIC ranges were found as 0.03-1 mu g/mL, 0.125 - >= 64 mu g/L, 0.03-2,mu g/mL and 0.015-2 mu g/mL for amphotericin B, fluconazole, voriconazole and caspofungin respectively. Conclusion: Proteinase and phospholipase activities can be suggested as important virulence factors in C. albicans infections, while the slime factor production seems to be more important in non-albicans Candida infections. In addition, amphotericin-B, voriconazole and caspofungin can be suggested to be effective on Candida strains and may be used as alternative drugs for treatment of infections caused by fluconazole-resistant strains.
Açıklama
Anahtar Kelimeler
Candida, C. Albicans, Virulence Factors, Antifungal Susceptibility
Kaynak
Acta Medica Mediterranea
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
30
Sayı
2
