Technical aspects and clinical limitations of sperm DNA fragmentation testing in male infertility a global survey, current guidelines, and expert recommendations

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dc.authorid0000-0002-1454-5672en_US
dc.contributor.authorSöğütdelen, Emrullah
dc.contributor.authorAgarwal, Ashok
dc.contributor.authorFarkouh, Ala'a
dc.contributor.authorSaleh, Ramadan
dc.contributor.authorHamoda, Taha Abo-Almagd Abdel-Meguid
dc.contributor.authorSalvio, Gianmaria
dc.date.accessioned2024-05-06T08:00:54Z
dc.date.available2024-05-06T08:00:54Z
dc.date.issued2024en_US
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description6. Indonesian Society of Andrological Urology (Gede Wirya Kusuma Duarsa, PhD, Indonesia) . 7. Indonesian Urological Association (Ponco Birowo, MD, PhD Indonesia; Gede Wirya Kusuma Duarsa, PhD, Indonesia; Fahmi Bahar, MD, Indonesia) . 8. Italian Society of Andrology and Sexual Medicine (Aldo E. Calogero, MD, Italy) . 9. Italian Society of Human Reproduction (Carlo Trotta, MD, Italy; Giovanni M. Colpi, MD, Italy; Lucia Rocco, PhD, Italy) . 10. Italian Society of Urology (Gian Maria Busetto, MD, PhD, Italy) . 11. Lebanese Society of Urology (Mohamad Moussa, MD, Lebanon) . 12. Malaysian Society of Andrology and the Study of the Aging Male (Christopher Ho, MD, Malaysia; Kay Seong, NGOO, MD, Malaysia) . 13. Malaysian Urological Association (Teng Aik Ong, MD, Malaysia) . 14. Mediterranean Society for Reproductive Medicine (Hassan Sallam, MD, PhD, Egypt) . 15. Middle East Society for Sexual Medicine (Amr El Meliegy, MD, Egypt) . 16. Romanian Association for Sexual Medicine (Catalina Zenoaga-Barbarosie, MSc, Romania) . 17. Saudi Andrology Group (Naif Alhathal, MD, Saudi Ara-bia) . 18. Society for Men's Health Singapore (King Chien; Joe Lee, MD, Singapore) . 19. Society of Egyptian Fellows and Members of the Royal College of Obstetricians and Gynecologists (Hassan Sallam MD, PhD, Egypt) . 20. Turkish Association of Urology (Arif Kalkanli, MD, Tur-key; Ates Kadioglu, MD, Turkey) . 21. Vietnam Society of Sexual Medicine (Ho Vinh Phuoc Nguyen, MD; Quang Nguyen, MD, PhD; Quang Tien Long Tran, MD; Tan V. Le, MD) .en_US
dc.description.abstractPurpose: Sperm DNA fragmentation (SDF) is a functional sperm abnormality that can impact reproductive potential, for which four assays have been described in the recently published sixth edition of the WHO laboratory manual for the exami-nation and processing of human semen. The purpose of this study was to examine the global practices related to the use of SDF assays and investigate the barriers and limitations that clinicians face in incorporating these tests into their practice. Materials and Methods: Clinicians managing male infertility were invited to complete an online survey on practices related to SDF diagnostic and treatment approaches. Their responses related to the technical aspects of SDF testing, current profes-sional society guidelines, and the literature were used to generate expert recommendations via the Delphi method. Finally, challenges related to SDF that the clinicians encounter in their daily practice were captured. Results: The survey was completed by 436 reproductive clinicians. Overall, terminal deoxynucleotidyl transferase deoxyuri-dine triphosphate Nick-End Labeling (TUNEL) is the most commonly used assay chosen by 28.6%, followed by the sperm chromatin structure assay (24.1%), and the sperm chromatin dispersion (19.1%). The choice of the assay was largely in-fluenced by availability (70% of respondents). A threshold of 30% was the most selected cut-off value for elevated SDF by 33.7% of clinicians. Of respondents, 53.6% recommend SDF testing after 3 to 5 days of abstinence. Although 75.3% believe SDF testing can provide an explanation for many unknown causes of infertility, the main limiting factors selected by respon-dents are a lack of professional society guideline recommendations (62.7%) and an absence of globally accepted references for SDF interpretation (50.3%). Conclusions: This study represents the largest global survey on the technical aspects of SDF testing as well as the barriers en-countered by clinicians. Unified global recommendations regarding clinician implementation and standard laboratory inter-pretation of SDF testing are crucial.en_US
dc.description.sponsorshipIndonesian Society of Andrological Urology; Indonesian Urological Association; Italian Society of Andrology and Sexual Medicine (Aldo E. Calogero, MD, Italy); Italian Society of Human Reproduction; Italian Society of Urology; Lebanese Society of Urology (Mohamad Moussa, MD, Lebanon); Malaysian Society of Andrology; Malaysian Urological Association; Mediterranean Society for Reproductive Medicine; Middle East Society for Sexual Medicine (Amr El Meliegy, MD, Egypt); Romanian Association for Sexual Medicine; Saudi Andrology Group; Society of Egyptian Fellows and Members of the Royal College of Obstetricians and Gynecologists (Hassan Sallam MD); Turkish Association of Urology; Vietnam Society of Sexual Medicine (Ho Vinh Phuoc Nguyen, MD)en_US
dc.identifier.citationAgarwal, A., Farkouh, A. A., Saleh, R., Hamoda, T. A. A. A. M., Salvio, G., Boitrelle, F., ... & Shah, R. (2024). Technical aspects and clinical limitations of sperm DNA fragmentation testing in male infertility: a global survey, current guidelines, and expert recommendations. The world journal of men's health, 42(1), 202.en_US
dc.identifier.doi10.5534/wjmh.230076
dc.identifier.endpage215en_US
dc.identifier.issn2287-4208
dc.identifier.issn2287-4690
dc.identifier.issue1en_US
dc.identifier.pmid37635341en_US
dc.identifier.scopus2-s2.0-85173813215en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage202en_US
dc.identifier.urihttp://dx.doi.org/10.5534/wjmh.230076
dc.identifier.urihttps://hdl.handle.net/20.500.12491/12117
dc.identifier.volume42en_US
dc.identifier.wosWOS:001063965600001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorSöğütdelen, Emrullah
dc.language.isoenen_US
dc.publisherKorean Soc Sexual Medicine & Andrologyen_US
dc.relation.ispartofWorld Journal Of Mens Healthen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDelphi Methoden_US
dc.subjectDiagnostic Testen_US
dc.subjectDNA Fragmentationen_US
dc.subjectInfertilityen_US
dc.subjectMaleen_US
dc.subjectSurveyen_US
dc.titleTechnical aspects and clinical limitations of sperm DNA fragmentation testing in male infertility a global survey, current guidelines, and expert recommendationsen_US
dc.typeArticleen_US

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