Frequency of MCP-1 (rs1024611) and CCR2 (rs1799864) gene polymorphisms, and its effect on gene expression level in patients with AgP

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dc.authorid0000-0003-4104-6462en_US
dc.authorid0000-0001-6100-322X
dc.authorid0000-0002-3519-1885
dc.contributor.authorGünpınar, Şadiye
dc.contributor.authorAlptekin, Nilgün Özlem
dc.contributor.authorUçar, Vasfiye Betül
dc.contributor.authorAcar, Hasan
dc.date.accessioned2021-06-23T19:45:40Z
dc.date.available2021-06-23T19:45:40Z
dc.date.issued2017
dc.departmentBAİBÜ, Diş Hekimliği Fakültesi, Periodontoloji Ana Bilim Dalıen_US
dc.description.abstractObjective: To identify the genetic risk markers of aggressive periodontitis (AgP), researchers focus on genetic components that regulate the immune response. Therefore the purpose of this study was to investigate genetic impact of monocyte chemoattractant protein (MCP)-1-2518 A/G and CC chemokine receptor 2 (CCR2) 190 G/A gene polymorphisms on AgP susceptibility and the effect of this polymorphism on MCP-1 gene expression in patients with AgP. Material and methods: A total of 215 subjects, 108 AgP and 107 periodontally healthy (H) were recruited in this cross-sectional study (NCT02817568). Gene polymorphisms of MCP -1-2518 A/G and CCR2-190 G/A were analyzed by a standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. MCP-1 messenger (m) RNA expression was measured using quantitative real-time (RT)-PCR in peripheral blood leukocytes from 26 AgP and 16 H controls. Threshold cycles (C-t) values were obtained from the RT-PCR analysis based on SYBR Green detection and data was normalized via triangle C-t. Results: There were no differences between AgP and H groups with regard to MCP-1 and CCR2 genotype distribution and allele frequencies (p > 0.05). In contrast, the MCP-1 mRNA expression levels were higher in homozygous "AA" control subjects than having G(+) genotype and AA homozygous AgP patients. Conclusions: It can be concluded that MCP-1 and CCR2 polymorphisms are not associated with AgP in Turkish population. Although in AgP patients, there was AA genotype with MCP -1 mRNA expression it can be speculated that gene expression levels in peripheral blood may not reflect the cytokine/chemokine levels of local tissues.en_US
dc.identifier.doi10.1016/j.archoralbio.2017.04.016
dc.identifier.endpage216en_US
dc.identifier.issn0003-9969
dc.identifier.issn1879-1506
dc.identifier.pmid28458180en_US
dc.identifier.scopus2-s2.0-85018314388en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage209en_US
dc.identifier.urihttps://doi.org/10.1016/j.archoralbio.2017.04.016
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9191
dc.identifier.volume80en_US
dc.identifier.wosWOS:000403729500029en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorGünpınar, Şadiye
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofArchives Of Oral Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAggressive Periodontitisen_US
dc.subjectCC Chemokine Receptor 2en_US
dc.subjectGene Expressionen_US
dc.subjectMonocyte Chemoattractant Protein-1en_US
dc.subjectRestriction Fragment Length Polymorphismen_US
dc.titleFrequency of MCP-1 (rs1024611) and CCR2 (rs1799864) gene polymorphisms, and its effect on gene expression level in patients with AgPen_US
dc.typeArticleen_US

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