Comparative de novo transcriptome analysis and random UV mutagenesis: Application in high biomass and astaxanthin production enhancement for Haematococcus pluvialis
Yükleniyor...
Dosyalar
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
BackgroundAstaxanthin is a natural carotenoid with strong antioxidant capacity. The high demand on astaxanthin by cosmetic, food, pharmaceutical and aquaculture industries promote its value in the biotechnological research. Haematococcus pluvialis Flotow 1844 has been characterized as one of the most promising species for natural astaxanthin biosynthesis. Even though H. pluvialis as an advantage in producing astaxanthin, its slow grow-yield limits usage of the species for large-scale production.Methods and ResultsIn this study we generated mutated H. pluvialis strain by using one-step random UV mutagenesis approach for higher biomass production in the green flagellated period and in turn higher astaxanthin accumulation in red stage per unit algae harvest. Isolated mutant strains were tested for the astaxanthin accumulation and yield of biomass. Among tested strains only mutant strain designated as only MT-3-7-2 showed a consistent and higher growth pattern, the rest had shown a fluctuated and then decreased growth rate than wild type. To demonstrate the phenotypical changes in MT-3-7-2 is associated with transcriptome, we carried out comparative analysis of transcriptome profiles between MT-3-7-2 and the wild type strains. De novo assembly was carried out to obtain the transcripts. Differential expression levels for the transcripts were evaluated by functional annotation analysis.ConclusionsData showed that increased biomass for the MT-3-7-2 strain was different from wild type with expression of transcripts upregulated in carbohydrate metabolism and downregulated in lipid metabolisms. Our data suggests a switching mechanism is enrolled between carbohydrate and lipid metabolism to regulate cell proliferation and stress responses.
Açıklama
This study was supported by Republic of Turkey Ministry of Agricultural & Forestry, General Directorate of Agricultural Research and Policies (TAGEM, Project No: 18/AR-GE/60) and Bolu Abant Izzet Baysal University Research Foundation (Project No: BAP - 2018.03.01.1317).& nbsp;
Anahtar Kelimeler
De Novo Transcriptomics, Astaxanthin, UV, Human Health, Mutants, Stress
Kaynak
Molecular Biology Report
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
50
Sayı
10
Künye
Telli, M., & Ünlü, E. S. (2023). Comparative de novo transcriptome analysis and random UV mutagenesis: application in high biomass and astaxanthin production enhancement for Haematococcus pluvialis. Molecular Biology Reports, 50(10), 8133-8143.
