In vitro cytotoxic, antibacterial, anti-inflammatory and antioxidant activities and phenolic content in wild-grown flowers of common daisy-A medicinal plant

dc.authorid0000-0001-5245-6294
dc.authorid0000-0001-9617-6673
dc.contributor.authorKarakaş, Fatma Pehlivan
dc.contributor.authorTürker, Arzu Uçar
dc.contributor.authorKarakaş, Alper
dc.contributor.authorMshvildadze, Vakhtang
dc.contributor.authorPichette, Andre
dc.date.accessioned2021-06-23T19:48:52Z
dc.date.available2021-06-23T19:48:52Z
dc.date.issued2017
dc.departmentBAİBÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.description.abstractBellis perennis L. is a medicinal plant in the family Compositae. It has been used as a remedy for wounds, rheumatism, eczema, eye diseases, inflammation and tonsillitis in folk medicine. In the present study, 19 different extracts and two fractions were obtained from wild-grown flowers, leaves and/or in vitro-grown leaves of common daisy by using different solvents and extraction methods. Biological activities of these extracts and fractions were assessed using selected bioassays: cytotoxic activity, disc diffusion assay, radical scavenging activity (DPPH), total phenolic content, oxygen radical absorbing capacity (ORAC) and 20,70-dichlorofluorescin-diacetate (DCFH-DA) cell-based assays. The cytotoxic activity of extracts and fractions was investigated against human lung carcinoma (A-549) and colon adenocarcinoma (DLD-1) cells. In vitro-grown leaf extracts showed the highest cytotoxic activity against selected cell lines. Moreover, n-butanol (n-BuOH) and ethyl acetate (EtOAc) fractions of flowers exerted high levels of cytotoxic activity. The MeOH extract and the EtOAc fraction of flowers exhibited broad-spectrum antibacterial activity against Streptococcus pyogenes, Staphylococcus aureus, Staphylococcus epidermidis, and Enterobacter cloacea. The strongest antioxidant activity was found in the EtOAc fraction of flowers with the highest amount of phenolic content and ORAC value. The MeOH extract of flowers showed strong anti-inflammatory activity on RAW 264.7 macrophages. The amount of the chosen 22 phenolic compounds in dichloromethane (DCM), MeOH extracts, n-BuOH and EtOAc fractions of field-grown flowers was detected using LC-ESI-MS/MS. The results of these studies support the potential use of B. perennis for wounds, rheumatism, inflammation, cancer and eye diseases. (C) 2016 Elsevier GmbH. All rights reserved.en_US
dc.identifier.doi10.1016/j.hermed.2016.11.003
dc.identifier.endpage39en_US
dc.identifier.issn2210-8033
dc.identifier.issn2210-8041
dc.identifier.scopus2-s2.0-85007481657en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage31en_US
dc.identifier.urihttps://doi.org/10.1016/j.hermed.2016.11.003
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9223
dc.identifier.volume8en_US
dc.identifier.wosWOS:000405677900006en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKarakaş, Fatma Pehlivan
dc.institutionauthorTürker, Arzu Uçar
dc.institutionauthorKarakaş, Alper
dc.language.isoenen_US
dc.publisherElsevier Gmbh, Urban & Fischer Verlagen_US
dc.relation.ispartofJournal Of Herbal Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBellis Perennisen_US
dc.subjectCytotoxicen_US
dc.subjectAntibacterialen_US
dc.subjectAntioxidanten_US
dc.subjectAnti-inflammatoryen_US
dc.subjectExtractionen_US
dc.titleIn vitro cytotoxic, antibacterial, anti-inflammatory and antioxidant activities and phenolic content in wild-grown flowers of common daisy-A medicinal planten_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
fatma-pehlivan-karakas.pdf
Boyut:
449 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text