Dye affinity cryogels for plasmid DNA purification

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dc.authorid0000-0002-9453-4462
dc.authorid0000-0003-0161-172X
dc.authorid0000-0003-3260-1639
dc.contributor.authorÇimen, Duygu
dc.contributor.authorYılmaz, Fatma
dc.contributor.authorPerçin, Işık
dc.contributor.authorTürkmen, Deniz
dc.contributor.authorDenizli, Adil
dc.date.accessioned2024-09-25T19:59:47Z
dc.date.available2024-09-25T19:59:47Z
dc.date.issued2015
dc.departmentBAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümü en_US
dc.description.abstractThe aim of this study is to prepare megaporous dye-affinity cryogel discs for the purification of plasmid DNA (pDNA) from bacterial lysate. Poly(hydroxyethyl methacrylate) [PHEMA] cryogel discs were produced by free radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) redox pair in an ice bath. Cibacron Blue F3GA was used as an affinity ligand (loading amount: 68.9 mu mol/g polymer). The amount of pDNA adsorbed onto the PHEMA-Cibacron Blue F3GA cryogel discs first increased and then reached a plateau value (i.e., 32.5 mg/g cryogel) at 3.0 mg/mL pDNA concentration. Compared with the PHEMA cryogel (0.11 mg/g cryogel), the pDNA adsorption capacity of the PHEMA-Cibacron Blue F3GA cryogel (32.4 mg/g polymer) was improved significantly due to the Cibacron Blue 3GA immobilization onto the polymeric matrix. pDNA adsorption amount decreased from 11.7 mg/g to 1.1 mg/g with the increasing of NaCl concentration. The maximum pDNA adsorption was achieved at 4 degrees C The overall recovery of pDNA was calculated as 90%. The PHEMA-Cibacron Blue F3GA cryogel discs could be used five times without decreasing the pDNA adsorption capacity significantly. The results show that the PHEMA-Cibacron Blue F3GA cryogel discs promise high selectivity for pDNA. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.msec.2015.06.041
dc.identifier.endpage324en_US
dc.identifier.issn0928-4931
dc.identifier.issn1873-0191
dc.identifier.pmid26249596en_US
dc.identifier.startpage318en_US
dc.identifier.urihttps://doi.org/10.1016/j.msec.2015.06.041
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13912
dc.identifier.volume56en_US
dc.identifier.wosWOS:000359873900038en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorYılmaz, Fatma
dc.institutionauthorid0000-0003-3260-1639
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofMaterials Science & Engineering C-Materials For Biological Applicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectPHEMAen_US
dc.subjectCryogelsen_US
dc.subjectCibacron Blue F3GAen_US
dc.subjectDNA purificationen_US
dc.subjectAffinity adsorptionen_US
dc.titleDye affinity cryogels for plasmid DNA purificationen_US
dc.typeArticleen_US

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