Protective effects of vitamin E forms (alpha-tocopherol, gamma-tocopherol and d-alpha-tocopherol polyethylene glycol 1000 succinate) on retinal edema during ischemia-reperfusion injury in the guinea pig retina

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dc.authorid0000-0003-2191-9297
dc.authorid0000-0003-2191-9297
dc.contributor.authorAydemir, Orhan
dc.contributor.authorÇelebi, Serdal
dc.contributor.authorYılmaz, Turgut
dc.contributor.authorYekeler, Hayrettin
dc.contributor.authorKükner, A.Sahap
dc.date.accessioned2021-06-23T18:54:35Z
dc.date.available2021-06-23T18:54:35Z
dc.date.issued2004
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractPurpose: The purpose of this study is to provide evidence that free radical damage is a component of retinal ischemia-reperfusion (I/R) injury, and to determine whether alpha-tocopherol, gamma-tocopherol and d-alpha-tocopherol polyethylene glycol 1000 succinate (TPGS) can protect the retina from this injury. Methods: The right eyes of 40 male guinea pigs weighing 500-600 g were used. The animals were randomly assigned to group 1 (control), group 2 (I/R), group 3 (I/R plus alpha-tocopherol), group 4 (I/R plus gamma-tocopherol) and group 5 (I/R plus TPGS). Groups 3, 4 and 5 received four subcutaneous injections at six-hour intervals for total dosage of 800 IU/kg alpha-tocopherol, 1000 IU/kg gamma-tocopherol and 750 IU/kg TPGS, respectively. The first dose of each substance was administered 5 minutes before retinal ischemia. Retinal ischemia was induced for 90 minutes, then followed by reperfusion for 24 hours. Injections of three substances were repeated at 6, 12 and 18 hours during reperfusion. The animals were killed at 24 hours of reperfusion. Sagittal sections of 4 ?m were cut and stained with hematoxylin and eosin for light microscopic evaluation. The average thickness (edema) of the inner plexiform layer for each eye was measured in sagittal sections near the optic nerve and expressed in microns. Results: All the three substances showed statistically significant protection against the formation of retinal edema during ischemia-reperfusion injury. The mean thickness of the inner plexiform layer were 15.0, 25.44, 19.81, 21.38 and 20.88 ?m in control, I/R, I/R plus alpha-tocopherol, I/R plus gamma-tocopherol and I/R plus TPGS groups, respectively. The results showed that the thickness of the inner plexiform layer in group 1 (control) was significantly lower than the other groups (p <0.001). The inner plexiform layer was thicker in the I/R group than with I/R plus alpha-tocopherol (p <0.001), I/R plus gamma-tocopherol (p <0.001) and I/R plus TPGS (p <0.01). The inner plexiform layer was not thicker in the I/R plus TPGS group than in the I/R plus alpha-tocopherol and I/R plus gamma-tocopherol groups. Compared to the I/R plus alpha-tocopherol group, the inner plexiform layer was significantly thicker in the I/R plus gamma-tocopherol group (p <0.01). Conclusions: The results from these experiments indicate that vitamin E forms have protective effects on the retina during retinal ischemia-reperfusion injury, but, the effects of alpha-tocopherol and TPGS appear to be much greater than that of gamma-tocopherol. © Springer 2006.en_US
dc.description.sponsorship101S014, 197S237en_US
dc.description.sponsorshipWe would like to thank Dr Andreas M. Papas from Eastman Chemical Company, USA for sending us free vitamin E forms. This work was supported by research grant numbers 97K120970 and 98K121420 from DPT, 101S014 and 197S237 was from TÜBİTAK, Turkey for the prevention of blindness.en_US
dc.identifier.doi10.1007/s10792-005-2034-z
dc.identifier.endpage289en_US
dc.identifier.issn0165-5701
dc.identifier.issue5-6en_US
dc.identifier.pmid16532291en_US
dc.identifier.scopus2-s2.0-33644908499en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage283en_US
dc.identifier.urihttps://doi.org/10.1007/s10792-005-2034-z
dc.identifier.urihttps://hdl.handle.net/20.500.12491/4494
dc.identifier.volume25en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKükner, A.Sahap
dc.institutionauthorÇelebi, Serdal
dc.language.isoenen_US
dc.publisherKluwer Academic Publishersen_US
dc.relation.ispartofInternational Ophthalmologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlpha-tocopherolen_US
dc.subjectGamma-tocopherolen_US
dc.subjectIschemia-reperfusion injuryen_US
dc.subjectRetinaen_US
dc.subjectTPGSen_US
dc.titleProtective effects of vitamin E forms (alpha-tocopherol, gamma-tocopherol and d-alpha-tocopherol polyethylene glycol 1000 succinate) on retinal edema during ischemia-reperfusion injury in the guinea pig retinaen_US
dc.typeArticleen_US

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