Microencapsulation of phages to analyze their demeanor in physiological conditions

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dc.authorid0000-0002-5765-3884en_US
dc.authorid0000-0002-6639-4212
dc.authorid0000-0003-1333-060X
dc.authorid0000-0001-7775-1871
dc.contributor.authorSoykut, Esra Acar
dc.contributor.authorTayyarcan, Emine Kübra
dc.contributor.authorEvran, Şefika
dc.contributor.authorBoyacı, İsmail Hakkı
dc.contributor.authorÇakır, İbrahim
dc.date.accessioned2021-06-23T19:50:37Z
dc.date.available2021-06-23T19:50:37Z
dc.date.issued2019
dc.departmentBAİBÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.description.abstractNowadays, phage therapy emerges as one of the alternative solutions to the problems arising from antibiotic resistance in pathogenic bacteria. Although phage therapy has been successfully applied both in vitro and in vivo, one of the biggest concerns in this regard is the stability of phages in body environment. Within the scope of this study, microencapsulation technology was used to increase the resistance of phages to physiological conditions, and the resulting microcapsules were tested in environments simulating body conditions. For this purpose, Bacillus subtilis, Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella Enteritidis), and Salmonella enterica subsp. enterica serovar Typhimurium (Salmonella Typhimurium) phages were isolated from different sources and then microencapsulated with 1.33%(w/v) sodiumalginate using a spray dryer to minimize the damage of physiological environment. Stability of microcapsules in simulated gastric fluid and bile salt presence was tested. As a consequence, the maximum titer decrease of microencapsulated phages after 2-h incubation was found to be 2.29 log unit for B. subtilis phages, 1.71 log unit for S. Enteritidis phages, and 0.60 log unit for S. Typhimurium phages, while free phages lost their viability even after a 15-min incubation. Similarly, microencapsulation was found to increase the stability of phages in the bile salt medium and it was seen that after 3 h of incubation, the difference between the titers of microencapsulated phages and free phages could reach up to 3 log unit.en_US
dc.identifier.doi10.1007/s12223-019-00688-1
dc.identifier.endpage763en_US
dc.identifier.issn0015-5632
dc.identifier.issn1874-9356
dc.identifier.issue6en_US
dc.identifier.pmid30756236en_US
dc.identifier.scopus2-s2.0-85061507113en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage751en_US
dc.identifier.urihttps://doi.org/10.1007/s12223-019-00688-1
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9826
dc.identifier.volume64en_US
dc.identifier.wosWOS:000510846800005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorSoykut, Esra Acar
dc.institutionauthorÇakır, İbrahim
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofFolia Microbiologicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMicroencapsulationen_US
dc.titleMicroencapsulation of phages to analyze their demeanor in physiological conditionsen_US
dc.typeArticleen_US

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