Hydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthan

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dc.authorid0000-0002-0335-2162en_US
dc.authorid0000-0002-2150-2726en_US
dc.authorid0000-0001-6951-1114en_US
dc.authorid0000-0002-7050-4082
dc.contributor.authorAlmutairi, Fahad M.
dc.contributor.authorErten, Tayyibe
dc.contributor.authorAdams, Gary G.
dc.contributor.authorHayes, Maria
dc.contributor.authorMcLoughlin, Padraig
dc.contributor.authorKök, Mehmet Şamil
dc.date.accessioned2021-06-23T19:42:05Z
dc.date.available2021-06-23T19:42:05Z
dc.date.issued2015
dc.departmentBAİBÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.description.abstractChitosan, a soluble polycationic derivative of insoluble chitin, has been widely considered for use in the food, cosmetic and pharmaceutical industries. Commercial ("C") and in-house laboratory ("L") prepared chitosan samples extracted from crustaceous shells with different molecular weight and degrees of acetylation (25% and 15%) were compared with regards to (i) weight-average molecular weight (M-w); (ii) sedimentation coefficient (s(20,w)(o)) distribution, and (iii) intrinsic viscosity ([eta]). These parameters were estimated using a combination of analytical ultracentrifugation (AUC), size exclusion chromatography coupled to multi-angle laser light scattering (SEC-MALS) and differential pressure viscometry. Polydisperse distributions were seen from sedimentation coefficient distributions and elution profiles from SEC-MALS. M-w values obtained for each sample by sedimentation equilibrium measurements were in excellent agreement with those obtained from SEC-MALS. Mark-Houwink-Kuhn-Sakurada (MHKS) and Wales van Holde analyses of the data all suggest a semi-flexible conformation. The principle of co-sedimentation was then used to monitor the interactions of the two different molecular weights of L chitosans with two polyanions, DNA and xanthan (another double helical high molecular weight molecule). Interactions were clearly observed and then quantified from the changes in the sedimentation coefficient distribution of the mixture compared to unmixed controls using sedimentation velocity. The interactions appeared to show a strong dependence on molecular weight. The relevance of this for DNA condensation applications is indicated. (C) 2014 Elsevier Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.carbpol.2014.09.090
dc.identifier.endpage366en_US
dc.identifier.issn0144-8617
dc.identifier.issn1879-1344
dc.identifier.pmid25817680en_US
dc.identifier.scopus2-s2.0-84925675020en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage359en_US
dc.identifier.urihttps://doi.org/10.1016/j.carbpol.2014.09.090
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8338
dc.identifier.volume122en_US
dc.identifier.wosWOS:000353249200046en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKök, Mehmet Şamil
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofCarbohydrate Polymersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSedimentation Distributionen_US
dc.subjectFlexibilityen_US
dc.subjectCo-Sedimentation, Double Helix, Multi-Gaussian Fiten_US
dc.titleHydrodynamic characterisation of chitosan and its interaction with two polyanions: DNA and xanthanen_US
dc.typeArticleen_US

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