Direct somatic embryogenesis from hypocotyl segments of Digitalis trojana Ivan and subsequent plant regeneration

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dc.authorid0000-0002-7631-823Xen_US
dc.authorid0000-0001-6262-2866en_US
dc.authorid0000-0003-2379-5363en_US
dc.authorid0000-0002-9324-747Xen_US
dc.contributor.authorVerma, Sandeep Kumar
dc.contributor.authorŞahin, Günce
dc.contributor.authorYücesan, Buhara
dc.contributor.authorEker, İsmail
dc.contributor.authorŞahbaz, Nevin
dc.contributor.authorGürel, Songül
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:29:00Z
dc.date.available2021-06-23T19:29:00Z
dc.date.issued2012
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractThis study describes, for the first time, an in vitro protocol for the direct development of somatic embryos and subsequent plant regeneration from hypocotyl segments excised from 21-days-old in vitro-germinated seedlings of Digitalis trojana Ivan (Helen of troy foxglove). Two sets of experiments were carried out, the first compared different concentrations of four cytokinins: N6-benzyladenine [BAP], 6-furfurylaminopurine [kinetin], 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea [TDZ], and 6-(4-hydroxy-3-methylbut-2-enylamino) purine [zeatin] I alone, while the second set tested TDZ or BAP combinations with IAA (indole-3-acetic acid), IBA (indole-3-butyric acid) or NAA (alpha-naphthalene acetic acid). In the first set of experiments, TDZ was found the most effective at 1.0 mg/l concentration, producing a mean of 10.7 somatic embryos per explant. In the second set, a combination of 1.0 mg/l TDZ with 0.5 mg/l IAA produced significantly more somatic embryos per explant (13.8 embryos) than with BAP (8.8 embryos). During subculture on growth regulator-free half-strength MS medium, somatic embryos gradually developed into plantlets. Regenerated plantlets were successfully transplanted and grown in a greenhouse environment. The efficient regeneration protocol reported here provides an important method of micropropagation of this plant. Furthermore, this protocol may be used for a large-scale production of cardenolides and genetic transformation of this valuable medicinal plant for its further improvement. (C) 2012 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.indcrop.2012.02.034
dc.identifier.endpage80en_US
dc.identifier.issn0926-6690
dc.identifier.issn1872-633X
dc.identifier.scopus2-s2.0-84858804205en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage76en_US
dc.identifier.urihttps://doi.org/10.1016/j.indcrop.2012.02.034
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7102
dc.identifier.volume40en_US
dc.identifier.wosWOS:000304744600011en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorVerma, Sandeep Kumar
dc.institutionauthorŞahin, Günce
dc.institutionauthorYücesan, Buhara
dc.institutionauthorEker, İsmail
dc.institutionauthorŞahbaz, Nevin
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofIndustrial Crops And Productsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDigitalis Trojanaen_US
dc.subjectHelen of Troy Foxgloveen_US
dc.subjectHypocotyl Segmentsen_US
dc.subjectDirect Somatic Embryogenesisen_US
dc.subjectTissue Cultureen_US
dc.titleDirect somatic embryogenesis from hypocotyl segments of Digitalis trojana Ivan and subsequent plant regenerationen_US
dc.typeArticleen_US

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