Enantioseparation of aromatic amino acids using CEC monolith with novel chiral selector, N-methacryloyl-L-histidine methyl ester

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dc.authorid0000-0002-3971-7725en_US
dc.authorid0000-0002-7692-0423en_US
dc.authorid0000-0001-7548-5741
dc.authorid0000-0003-3260-1639
dc.contributor.authorAydoğan, Cemil
dc.contributor.authorYılmaz, Fatma
dc.contributor.authorÇimen, Duygu
dc.contributor.authorUzun, Lokman
dc.contributor.authorDenizli, Adil
dc.date.accessioned2021-06-23T19:34:28Z
dc.date.available2021-06-23T19:34:28Z
dc.date.issued2013
dc.departmentBAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümüen_US
dc.description.abstractA new type of polymethacrylate-based monolithic column with chiral stationary phase was prepared for the enantioseparation of aromatic amino acids, namely d,l-phenylalanine, d,l-tyrosine, and d,l-tryptophan by CEC. The monolithic column was prepared by in situ polymerization of butyl methacrylate (BMA), N-methacryloyl-l-histidine methyl ester (MAH), and ethylene dimethacrylate (EDMA) in the presence of porogens. The porogen mixture included DMF and phosphate buffer. MAH was used as a chiral selector. FTIR spectrum of the polymethacrylate-based monolith showed that MAH was incorporated into the polymeric structure via in situ polymerization. Some experimental parameters including pH, concentration of the mobile phase, and MAH concentration with regard to the chiral CEC separation were investigated. Single enantiomers and enantiomer mixtures of the amino acids were separately injected into the monolithic column. It was observed that l-enantiomers of aromatic amino acids migrated before d-enantiomers. The reversal enantiomer migration order for tryptophan was observed upon changing of pH. Using the chiral monolithic column (100 m id and 375 m od), the best chiral separation was performed in 35:65% ACN/phosphate buffer (pH 8.0, 10mM) with an applied voltage of 12 kV in CEC. SEM images showed that the chiral monolithic column has a continuous polymeric skeleton and large through-pore structure.en_US
dc.identifier.doi10.1002/elps.201200125
dc.identifier.endpage1914en_US
dc.identifier.issn0173-0835
dc.identifier.issn1522-2683
dc.identifier.issue13en_US
dc.identifier.pmid23592237en_US
dc.identifier.scopus2-s2.0-84879821059en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1908en_US
dc.identifier.urihttps://doi.org/10.1002/elps.201200125
dc.identifier.urihttps://hdl.handle.net/20.500.12491/7520
dc.identifier.volume34en_US
dc.identifier.wosWOS:000322112200009en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorYılmaz, Fatma
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofElectrophoresisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmino Acid Enantioseparationen_US
dc.subjectCECen_US
dc.subjectChiral Selectoren_US
dc.subjectMonolithen_US
dc.subjectReversal EMOen_US
dc.titleEnantioseparation of aromatic amino acids using CEC monolith with novel chiral selector, N-methacryloyl-L-histidine methyl esteren_US
dc.typeArticleen_US

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