Plantlet regeneration from young leaf segments of Curaua (Ananas Erectifolius), an Amazon species

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dc.contributor.authorMoreira, Carolina Mariane
dc.contributor.authorAndrade, Helena Botelho De
dc.contributor.authorBertolucci, Suzan Kelly Vilela
dc.contributor.authorLameira, Osmar Alves
dc.contributor.authorMohammed, Aliyu
dc.date.accessioned2021-06-23T18:40:11Z
dc.date.available2021-06-23T18:40:11Z
dc.date.issued2016
dc.departmentBAİBÜ, Lisansüstü Eğitim Enstitüsü, Fen Bilimleri, Biyoloji Ana Bilim Dalıen_US
dc.description.abstractThe present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6-trichloropicolinic acid (picloram; 48.57%), 2,4-D (40%), or TDZ (35.71%). However, only calli generated in treatments containing TDZ or N6-isopentenyladenine (2ip) were able to develop shoots (maximum 35.71% and 14.28%, respectively). A combination of TDZ and 2,4-D improved callus induction (60.0%) but did not increase shooting response. However, both callusing and shooting response increased when 10 days of pulse treatment with TDZ and 2,4-D was applied (66.83% and 48.7%, respectively). Rooted plantlets that exhibited normal growth and development were acclimatized in a greenhouse and had a survival rate of 95%.en_US
dc.identifier.endpage1234en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85007398072en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1227en_US
dc.identifier.trdizinid214776en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWpFME56YzJOZz09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/3000
dc.identifier.volume40en_US
dc.identifier.wosWOS:000391393100011en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorMohammed, Aliyu
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - İdari Personel ve Öğrencien_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTananas Erectifoliusen_US
dc.subjectCallus Induction
dc.subjectIn Vitro Regeneration
dc.subjectPulse Treatment
dc.subjectThidiazuron
dc.titlePlantlet regeneration from young leaf segments of Curaua (Ananas Erectifolius), an Amazon speciesen_US
dc.typeArticleen_US

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