Production of doubled haploids in sugar beet (beta vulgaris): An efficient method by a multivariate experiment

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dc.authorid0000-0001-5291-1824en_US
dc.authorid0000-0002-1205-268Xen_US
dc.authorid0000-0003-2379-5363en_US
dc.authorid0000-0002-4005-2476en_US
dc.authorid0000-0001-6262-2866en_US
dc.contributor.authorPazuki, Arman
dc.contributor.authorAflaki, Fatemeh
dc.contributor.authorGürel, Songül
dc.contributor.authorErgül, Ali
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:50:09Z
dc.date.available2021-06-23T19:50:09Z
dc.date.issued2018
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractThe present paper describes a detailed study of a highly efficient protocol to multiply the number of haploids in sugar beet production and subsequent chromosome doubling. The protocol involves an experiment investigating factorial interactions between cold pretreatment, seven genotypes of sugar beet, and kinetin to improve haploid embryo induction. In addition, the effects of color of ovules and flower bud position on haploid embryo induction were investigated. After subjecting the data to analysis of variance or Student's t test (P < .05), the effect sizes of the independent variables were also estimated. Cold pretreatment was effective in stimulating the ovules. The haploid embryo induction rate for 1-week cold pretreated ovules (9.01%) was higher than that of freshly cultured ones (6.15%). In comparison with hormone-free medium (5.16%), the gynogenesis rate for the media supplemented with 0.05 or 0.5 mg L-l kinetin increased to 7.58 and 10.05%, respectively. The genotype responses were significantly different. Interactions of kinetin x cold pretreatment, genotype x hormonal treatment, genotype x cold pretreatment, and the three-way interaction were statistically significant. Moreover, the main effects of flower bud position, ovule color, and comma-form ovule on gynogenic response were significant. After investigating the effect of 5 g L-l colchicine for 3, 5, or 7 min on one genotype's (SG2) specimens, all the haploid plantlets from the other genotypes were treated for 5 min as the best treatment. The paper discusses interactions of the factors, which may be interesting for others aiming to breed doubled haploid sugar beet or possibly other related plant species.en_US
dc.identifier.doi10.1007/s11240-017-1313-5
dc.identifier.endpage97en_US
dc.identifier.issn0167-6857
dc.identifier.issn1573-5044
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85029530329en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage85en_US
dc.identifier.urihttps://doi.org/10.1007/s11240-017-1313-5
dc.identifier.urihttps://hdl.handle.net/20.500.12491/9715
dc.identifier.volume132en_US
dc.identifier.wosWOS:000418824200006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPlant Cell Tissue And Organ Cultureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSugar Beeten_US
dc.subjectGynogenesisen_US
dc.subjectHaploiden_US
dc.subjectBeta Vulgarisen_US
dc.subjectKinetinen_US
dc.subjectOvuleen_US
dc.titleProduction of doubled haploids in sugar beet (beta vulgaris): An efficient method by a multivariate experimenten_US
dc.typeArticleen_US

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