Cytotoxic, anti-inflammatory and antioxidant activities of four different extracts of Galega officinalis L (goat's rue)

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dc.authorid0000-0001-5245-6294
dc.authorid0000-0001-9617-6673
dc.contributor.authorKarakaş, Fatma Pehlivan
dc.contributor.authorTürker, Arzu Uçar
dc.contributor.authorKarakaş, Alper
dc.contributor.authorMshvildadze, Vakhtang
dc.date.accessioned2021-06-23T19:43:40Z
dc.date.available2021-06-23T19:43:40Z
dc.date.issued2016
dc.departmentBAİBÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractPurpose: To evaluate the cytotoxic, anti-inflammatory and antioxidant activities of four different solvent extracts obtained from the aerial parts of Galega officinalis L. Methods: The hexane, DCM, methanol and water extracts of G. officinalis were successively obtained by soxhlet extraction method. The cytotoxic activity of the extracts was assessed against human lung carcinoma (A-549), human colorectal adenocarcinoma (HT-29), human brain glioblastoma (U-87), and colon adenocarcinoma (DLD-1) by Resazurine test. The antioxidant activity of extracts were determined by Folin-Ciocalteau, oxygen radical absorbing capacity (ORAC), and 2'.7'-dichlorofluorescin-diacetate (DCFH-DA) cell-based assay while their anti-inflammatory activity was determined by nitric oxide (NO) assay. Results: DCM extract showed strong cytotoxic activity against lung adenocarcinoma and brain glioblastoma cell lines, with IC50 (concentration inhibiting 50 % of cell growth) values of 11 +/- 0.4 and 16 +/- 3 mu g/mL, respectively. The hexane extract showed moderate anticancer activity against the same cell lines (59 +/- 13 and 63 +/- 16 mu g/mL, respectively). DCM extract also showed significant anti-inflammatory activity, inhibiting NO release by 86.7 % at 40 mu g/mL in lipopolysaccharide (LPS) - stimulated murine RAW 264.7 macrophages. Of all test extracts, the methanol extract of G. officinalis showed the highest antioxidant activity with 2.33 +/- 0.09 mu mol Trolox/mg, 7.10 +/- 0.9 g tannic acid equivalent (TAE), and IC50 of 44 +/- 4 mu g/mL. Conclusion: The findings of this study suggest that DCM extract may possess anticancer effect against lung adenocarcinoma and brain glioblastoma, as well as serve as an anti-inflammatory agent.en_US
dc.identifier.doi10.4314/tjpr.v15i4.12
dc.identifier.endpage757en_US
dc.identifier.issn1596-5996
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84964931703en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage751en_US
dc.identifier.urihttps://doi.org/10.4314/tjpr.v15i4.12
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8828
dc.identifier.volume15en_US
dc.identifier.wosWOS:000376755700012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorKarakaş, Fatma Pehlivan
dc.institutionauthorTürker, Arzu Uçar
dc.institutionauthorKarakaş, Alper
dc.language.isoenen_US
dc.publisherPharmacotherapy Groupen_US
dc.relation.ispartofTropical Journal Of Pharmaceutical Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGalega Officinalis Len_US
dc.subjectBiological Activityen_US
dc.subjectBioassayen_US
dc.subjectAnticanceren_US
dc.subjectLung Adenocarcinomaen_US
dc.subjectBrain Glioblastomaen_US
dc.subjectGoat's Rueen_US
dc.titleCytotoxic, anti-inflammatory and antioxidant activities of four different extracts of Galega officinalis L (goat's rue)en_US
dc.typeArticleen_US

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