New Detection Methods for Cryphonectria Hypovirus 1 (CHV1) through SYBR Green-Based Real-Time PCR and Loop-Mediated Isothermal Amplification (LAMP)

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dc.authoridMorca, Ali Ferhan/0000-0002-7480-922X
dc.authoridDervis, Sibel/0000-0002-4917-3813
dc.authoridOZER, Goksel/0000-0002-3385-2520
dc.contributor.authorCelik, Ali
dc.contributor.authorCakar, Deniz
dc.contributor.authorDervis, Sibel
dc.contributor.authorMorca, Ali Ferhan
dc.contributor.authorSimsek, Secil Akilli
dc.contributor.authorRomon-Ochoa, Pedro
dc.contributor.authorOzer, Goksel
dc.date.accessioned2024-09-25T19:57:26Z
dc.date.available2024-09-25T19:57:26Z
dc.date.issued2024
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractSome mycoviruses can be considered as effective biocontrol agents, mitigating the impact of phytopathogenic fungi and consequently reducing disease outbreaks while promoting plant health. Cryphonectria parasitica, the causal agent of chestnut blight and a highly destructive pathogen, experienced a notable decrease in its virulence with the identification of cryphonectria hypovirus 1 (CHV1), a naturally occurring biocontrol agent. In this study, two innovative diagnostic protocols designed for the accurate and efficient detection of CHV1 are introduced. The ORF A and ORF B regions of CHV1 are targeted by these techniques, which employ colorimetric loop-mediated isothermal amplification (LAMP) with 2 Colorimetric LAMP Master Mix and real-time quantitative PCR (qPCR) with SYBR Green chemistry, respectively. The LAMP assay presents a discernible color transition, changing from pink to yellow after a 35 min incubation period. Comparative analysis, when assessed against two established reverse transcription-PCR (RT-PCR) techniques, reveals a significant enhancement in sensitivity for both the LAMP approach, which offers a tenfold increase, and the qPCR method, which showcases a remarkable 100-fold sensitivity improvement. Throughout the comparison phase, it was evident that the RT-PCR, LAMP, and qPCR procedures displayed superior performance compared to the Bavendamm test, relying on phenol oxidase activity, effectively distinguishing hypovirulent strains. Consequently, this study introduces two pioneer diagnostic assays for highly sensitive CHV1 detection, representing a substantial advancement in the realm of CHV1 surveillance techniques. These methodologies hold significant promise for enhancing research endeavors in the domain of the biological control of C. parasitica.en_US
dc.description.sponsorshipTURKISH project; British Department of Environment, Food and Rural Affairs [Th32324]; Turkish and British projectsen_US
dc.description.sponsorshipThis research was funded by TURKISH project and the British Department of Environment, Food and Rural Affairs, DEFRA-funded projects Th32324 and 3_1 (Control of chestnut blight). The APC was co-funded by both Turkish and British projects.en_US
dc.identifier.doi10.3390/v16081203
dc.identifier.issn1999-4915
dc.identifier.issue8en_US
dc.identifier.pmid39205177en_US
dc.identifier.scopus2-s2.0-85202534507en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/v16081203
dc.identifier.urihttps://hdl.handle.net/20.500.12491/13413
dc.identifier.volume16en_US
dc.identifier.wosWOS:001307436400001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofViruses-Baselen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectCHV1en_US
dc.subjectcolorimetricen_US
dc.subjectLAMPen_US
dc.subjectSYBR Greenen_US
dc.subjectqPCRen_US
dc.titleNew Detection Methods for Cryphonectria Hypovirus 1 (CHV1) through SYBR Green-Based Real-Time PCR and Loop-Mediated Isothermal Amplification (LAMP)en_US
dc.typeArticleen_US

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