In vitro plant regeneration of Ocimum basilicum L. is accelerated by zinc sulfate

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dc.authorid0000-0003-0616-9715en_US
dc.authorid0000-0001-6262-2866en_US
dc.authorid0000-0002-9324-747Xen_US
dc.authorid0000-0003-0060-259X
dc.contributor.authorVerma, Sandeep Kumar
dc.contributor.authorŞahin, Günce
dc.contributor.authorDas, Ashok Kumar
dc.contributor.authorGürel, Ekrem
dc.date.accessioned2021-06-23T19:43:54Z
dc.date.available2021-06-23T19:43:54Z
dc.date.issued2016
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractThis article describes a fast, simple, and efficient plant regeneration protocol for Ocimum basilicum L. Two sets of experiments were performed. The first set was performed on Murashige and Skoog (MS) medium lacking zinc sulfate heptahydrate (ZnSO4 center dot 7H(2)O) and containing varying concentrations of indole-3-acetic acid (IAA). The second set used three different concentrations (8.6, 12.9, and 17.2 mg L-1) of ZnSO4 center dot 7H(2)O along with varying concentrations of IAA. In the first set, without zinc sulfate, an IAA concentration of 1.0 mg L-1 (MS4) was found to be most effective, producing a mean of 12.6 roots per hypocotyl explant, while shoots were not produced. In the second set, with zinc sulfate, a combination of 12.9 mg L-1 ZnSO4 center dot 7H(2)O + 1.0 mg L-1 IAA (MS11) produced significantly more shoots per explant (15 shoots) than a combination of 12.9 mg L-1 ZnSO4 center dot 7H(2)O + 0.5 mg L-1 IAA (MS10), which produced only six shoots. Later, the plantlets were successfully acclimatized (100%) and finally transferred to the greenhouse (ex vitro). In the O. basilicum plants grown using MS11 medium, total phenolic content and rosmarinic acid content were estimated from stem, shoot tip, and old leaf tissue of in vitro plantlets, ex vitro plantlets, and flowering plants. The highest amounts of total phenolic content (131.8 mg gallic acid equivalent g(-1) DW) and rosmarinic acid (13.0 mg g(-1) DW) were obtained in the old leaf tissue at flowering time. This rapid regeneration protocol for O. basilicum L. represents a major improvement over the conventional protocols for plant regeneration and propagation of this species.en_US
dc.identifier.doi10.1007/s11627-015-9739-0
dc.identifier.endpage27en_US
dc.identifier.issn1054-5476
dc.identifier.issn1475-2689
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84958853430en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage20en_US
dc.identifier.urihttps://doi.org/10.1007/s11627-015-9739-0
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8877
dc.identifier.volume52en_US
dc.identifier.wosWOS:000370819900003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorVerma, Sandeep Kumar
dc.institutionauthorŞahin, Günce
dc.institutionauthorGürel, Ekrem
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofIn Vitro Cellular & Developmental Biology-Planten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOcimum Basilicumen_US
dc.subjectShoot Regenerationen_US
dc.subjectTotal Phenolic Contenten_US
dc.subjectRosmarinic Acid Contenten_US
dc.titleIn vitro plant regeneration of Ocimum basilicum L. is accelerated by zinc sulfateen_US
dc.typeArticleen_US

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