Analysis of metabolic variations throughout growth and development of adventitious roots in Silybum marianum L. (Milk thistle), a medicinal plant

Basit Öğe Kaydı
dc.authorid0000-0002-6529-2134en_US
dc.authorid0000-0002-7994-4244en_US
dc.authorid0000-0002-6786-3973
dc.contributor.authorKhan, Mubarak Ali
dc.contributor.authorAbbasi, Bilal Haider
dc.contributor.authorShah, Naseer Ali
dc.contributor.authorYücesan, Buhara
dc.contributor.authorAli, Huma
dc.date.accessioned2021-06-23T19:37:22Z
dc.date.available2021-06-23T19:37:22Z
dc.date.issued2015
dc.departmentBAİBÜ, Ziraat Fakültesi, Tohum Bilimi ve Teknolojisi Bölümüen_US
dc.description.abstractSilybum marianum L. is a medicinal plant used in the treatment for jaundice and liver diseases. In this study, an adventitious root culture was developed for the production of health promoting phytochemicals. Adventitious roots were induced from nodal explants on solid Murashige and Skoog medium supplemented with 1.0 mg l(-1) of alpha-Naphthalene acetic acid. Growth kinetics of the roots was investigated every week, for 8 weeks of culture period. Highest fresh biomass formation (153 mg l(-1)) was observed in 6-week old cultures. Adventitious roots were harvested from different growth stages as control (CTR), lag phase (LAG), logarithmic phase (LOG) or stationary phase (STN). Metabolite profiling of the samples was investigated using electro spray ionization time of flight mass spectrometry. Significant phenylpropanoids such as cinnamic acid and di-hydro kaempferol were predominantly found in LOG phase, whereas the highest amount of malonic acid was detected in STN as compared to other growth phases. More sucrose content was detected in CTR, while the tryptophan content was higher in LOG phase. Among the vital fatty acids, prostaglandin A1 and phenyl acetic acid were at highest levels in STN phase. However, more brassicasterols were observed in LAG phase than other growth phases. Punicic acid and lignan pinoresinol were detected abundantly in the LOG phase. Biochemical characterization revealed significant correlations between silymarin content and DPPH as well as TPC and TFC in the growth curve. Interestingly, among all growth stages there was no correlation of PAL activity with TFC and silymarin content.en_US
dc.identifier.doi10.1007/s11240-015-0854-8
dc.identifier.endpage510en_US
dc.identifier.issn0167-6857
dc.identifier.issn1573-5044
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84947490092en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage501en_US
dc.identifier.urihttps://doi.org/10.1007/s11240-015-0854-8
dc.identifier.urihttps://hdl.handle.net/20.500.12491/8148
dc.identifier.volume123en_US
dc.identifier.wosWOS:000365216300006en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorYücesan, Buhara
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPlant Cell Tissue And Organ Cultureen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSilybum Marianum L.en_US
dc.subjectSilymarinen_US
dc.subjectAdventitious Rootsen_US
dc.subjectMass Spectrometryen_US
dc.subjectPlant Secondary Metabolitesen_US
dc.titleAnalysis of metabolic variations throughout growth and development of adventitious roots in Silybum marianum L. (Milk thistle), a medicinal planten_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
mubarak-ali-khan.pdf
Boyut:
1.14 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam metin/Full text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Tohum Bilimi ve Teknolojisi Bölümü Koleksiyonu